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Literature summary for 2.4.1.198 extracted from

  • Watanabe, R.; Murakami, Y.; Marmor, M.; Inoue, N.; Maeda, Y.; Hino, J.; Kangawa, K.; Julius, M.; Kinoshita, T.
    Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2 (2000), EMBO J., 19, 4402-4411.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.198

Activating Compound

Activating Compound Comment Organism Structure
component 2 of dolichyl-phosphate-mannose synthase i.e. DPM2 Homo sapiens
component 2 of dolichyl-phosphate-mannose synthase DPM2 protein binds directly to the enzyme complex and enhances the enzyme activity, regulatory role Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of PIG-P in enzyme deficient mouse mutant T cell line can complement and restore the enzyme activity Homo sapiens
overexpression of FLAG- and GST-double tagged PIG-A in human B lymphoblastoid JY5 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
endoplasmic reticulum
-
 Mus musculus 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
 enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H Homo sapiens
additional information
-
 enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H, PIG-P is absolutely required ?
-
 ?
additional information Mus musculus enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H, PIG-P is absolutely required ?
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Homo sapiens involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Mus musculus involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P57054 gene PIG-P
-
Homo sapiens Q9BRB3
-
-
Mus musculus Q9JHG1 gene PIG-P
-

Purification (Commentary)

Purification (Comment) Organism
purification of GPI1 by overexpression of PIG-A in JY5 cells and isolation of the complex by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Homo sapiens ?
-
 ?
additional information enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H, PIG-P is absolutely required Homo sapiens ?
-
 ?
additional information enzyme is active in a complex of at least 5 proteins, termed GPI1, PIG-P, PIG-A, PIG-C and PIG-H, PIG-P is absolutely required Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Homo sapiens UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Mus musculus UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Homo sapiens UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Mus musculus UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?

Subunits

Subunits Comment Organism
More structure of enzyme complex, overview Homo sapiens
More structure of enzyme complex, overview Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Mus musculus