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Literature summary for 2.4.1.19 extracted from

  • Tomita, K.; Kaneda, M.; Kawamura, K.; Nakanishi, K.
    Purification and properties of a cyclodextrin glucanotransferase from Bacillus autolyticus 11149 and selective formation of beta-cyclodextrin (1993), J. Ferment. Bioeng., 75, 89-92.
No PubMed abstract available
Search for more literature for 2.4.1.19

Activating Compound

Activating Compound Comment Organism Structure
(CH3COO)2Pb relative activity 103% Bacillus autolyticus
2-mercaptoethanol relative activity 101% Bacillus autolyticus
dithiothreitol relative activity 101% Bacillus autolyticus
ethanol
-
 Geobacillus stearothermophilus
ethanol
-
 Bacillus sp. (in: Bacteria)
ethanol
-
 Niallia circulans
ethanol
-
 Paenibacillus macerans
ethanol enhances specificity and yield of beta-cyclodextrin production Bacillus autolyticus
Triton X-100 enhances specificity and yield of beta-cyclodextrin production Bacillus autolyticus

Application

Application Comment Organism
synthesis
-
 Geobacillus stearothermophilus
synthesis
-
 Bacillus sp. (in: Bacteria)
synthesis
-
 Niallia circulans
synthesis
-
 Paenibacillus macerans
synthesis used in industrial applications Bacillus autolyticus

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
 Bacillus autolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
-
 Geobacillus stearothermophilus
68000
-
-
 Bacillus sp. (in: Bacteria)
68000
-
-
 Paenibacillus macerans
68000
-
 gel filtration Bacillus autolyticus
70000
-
-
 Geobacillus stearothermophilus
70000
-
 SDS-PAGE Bacillus autolyticus

Organism

Organism UniProt Comment Textmining
Bacillus autolyticus
-
 11149
-
Bacillus autolyticus 11149
-
 11149
-
Bacillus sp. (in: Bacteria)
-
 AL-6
-
Bacillus sp. (in: Bacteria) AL-6
-
 AL-6
-
Geobacillus stearothermophilus
-
-
-
Klebsiella oxytoca
-
 formerly Klebsiella pneumoniae M5a1
-
Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
Paenibacillus macerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus
-
 Bacillus sp. (in: Bacteria)
-
 Paenibacillus macerans
-
 Bacillus autolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
additional information
-
-
 Niallia circulans
additional information
-
-
 Paenibacillus macerans
additional information
-
 332.5 units/mg, 1 unit is the amount of enzyme which decreases 10% of the absorbance at 700 nm per min Bacillus autolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Geobacillus stearothermophilus ?
-
 ?
additional information
-
 Niallia circulans ?
-
 ?
additional information
-
 Paenibacillus macerans ?
-
 ?
additional information sorbitol, mannitol, xylulose, galactose, fructose, lactose, glycerol and arabinose are not acceptors Bacillus autolyticus ?
-
 ?
additional information sorbitol, mannitol, xylulose, galactose, fructose, lactose, glycerol and arabinose are not acceptors Bacillus autolyticus 11149 ?
-
 ?
soluble starch + D-glucose
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
soluble starch + D-glucose
-
 Paenibacillus macerans cyclodextrins
-
 ?
soluble starch + D-glucose
-
 Bacillus autolyticus cyclodextrins
-
 r
soluble starch + D-glucose
-
 Bacillus autolyticus 11149 cyclodextrins
-
 r
soluble starch + D-maltose
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
soluble starch + D-maltose
-
 Niallia circulans cyclodextrins
-
 r
soluble starch + D-maltose
-
 Paenibacillus macerans cyclodextrins
-
 r
soluble starch + D-maltose
-
 Bacillus autolyticus cyclodextrins
-
 r
soluble starch + D-maltose
-
 Bacillus autolyticus 11149 cyclodextrins
-
 r
soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) Schardinger beta-dextrin
-
 ?
soluble starch + glycosyl acceptor ATCC 21783 Niallia circulans Schardinger beta-dextrin
-
 r
soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 Schardinger beta-dextrin
-
 ?
soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor potato starch, small amounts of alpha- and gamma-cyclodextrin Bacillus autolyticus beta-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 beta-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor potato starch, small amounts of alpha- and gamma-cyclodextrin Bacillus autolyticus 11149 beta-cyclodextrin
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Bacillus sp. (in: Bacteria)
monomer
-
 Niallia circulans
monomer
-
 Paenibacillus macerans
monomer 1 * 70000, SDS-PAGE Bacillus autolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus stearothermophilus
60
-
-
 Bacillus sp. (in: Bacteria)
60
-
-
 Niallia circulans
60
-
-
 Paenibacillus macerans
60
-
-
 Bacillus autolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Bacillus autolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9
-
 Geobacillus stearothermophilus
5.5 9
-
 Niallia circulans
5.5 9 stable for 12 h at 40°C, below 40°C stable for 1 h at pH 6.0 Bacillus autolyticus