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Literature summary for 2.4.1.189 extracted from

  • Schulz, M.; Weissenböck, G.
    Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale (1988), Phytochemistry, 27, 1261-1267.
No PubMed abstract available
Search for more literature for 2.4.1.189

General Stability

General Stability Organism
freezing without glycerol, complete inactivation Secale cereale

Inhibitors

Inhibitors Comment Organism Structure
luteolin 0.14 mM, 60% inhibition Secale cereale
UDP strong inhibition Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 luteolin
-
 Secale cereale
0.012
-
 UDPglucuronic acid
-
 Secale cereale

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol probably Secale cereale 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.25 mM, 25% stimulation Secale cereale
Mg2+ up to 1 mM, 20% stimulation Secale cereale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
 gel filtration Secale cereale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucuronic acid + luteolin Secale cereale first enzyme in the biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-glucuronide
-
 ir

Organism

Organism UniProt Comment Textmining
Secale cereale
-
 rye
-

Purification (Commentary)

Purification (Comment) Organism
ultrogel AcA 44, DEAE-cellulose, partial purification Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16
-
-
 Secale cereale

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-beta-D-glucoside
-
 ?
UDPgalactose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-galactoside
-
 ?
UDPglucuronic acid + apigenin 38% of activity compared to luteolin Secale cereale UDP + apigenin 7-O-glucuronide
-
 ?
UDPglucuronic acid + luteolin
-
 Secale cereale UDP + luteolin 7-O-glucuronide
-
 ir
UDPglucuronic acid + luteolin first enzyme in the biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-glucuronide
-
 ir
UDPxylose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-xyloside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 50% of maximal activity at pH 5.6 Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
5.6 9 50% of maximal activity at pH 5.6 and pH 9.0 Secale cereale

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.26
-
 UDP
-
 Secale cereale