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Literature summary for 2.4.1.186 extracted from
- Expression and purification of functional human glycogen synthase-1:glycogenin-1 complex in insect cells (2015), Protein Expr. Purif., 108, 23-29.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GYG1, recombinant expression of functional GST-tagged human muscle glycogen synthase in complex with human glycogenin-1 in Spodoptera frugiperda Sf9 cells using a bicistronic pFastBac-Dual expression vector and baculovirus transfection | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P46976 | gene GYG1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the degree of glycosylation of the enzyme is modest | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged glycogen synthase in complex with glycogenin-1 from Sf9 insect cells by glutathione affinity chromatography and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + glycogenin-1 | self-glucosylation of the glycosyltransferase glycogenin-1 | Homo sapiens | UDP + alpha-D-glucosylglycogenin-1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogenin-1 | - |
Homo sapiens |
| GN1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the recombinant glycogen synthase-glycogenin-1 complex GYS1:GN1 is functional and exhibits both allosteric and phospho-dependent regulatio, activation of GYS1:GN1 complex by GYS1 dephosphorylationn | Homo sapiens |
| physiological function | glycogenin-1 is a constitutively active enzyme so does not represent a point of regulation | Homo sapiens |
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Release 2023.1 (January 2023)
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