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Literature summary for 2.4.1.186 extracted from

  • Bazan, S.; Issoglio, F.M.; Carrizo, M.E.; Curtino, J.A.
    The intramolecular autoglucosylation of monomeric glycogenin (2008), Biochem. Biophys. Res. Commun., 371, 328-332.
    View publication on PubMed
Search for more literature for 2.4.1.186

Cloned(Commentary)

Cloned (Comment) Organism
preparation of a truncated already glucosylated His-tag fusion protein in Escherichia coli ER2566, expression of the truncated non-glucosylated His-tag fusion protein in Escherichia coli CGSC 4997 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 non-glycosylated truncated protein, gel filtration of a 0.0005 mM solution Oryctolagus cuniculus
38000
-
 1 * 38000, native enzyme, 50-70% of the activity of the dimer Oryctolagus cuniculus
38000
-
 2 * 38000, native enzyme Oryctolagus cuniculus
59000
-
 non-glycosylated truncated protein, gel filtration of a 0.02 mM solution Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + glycogenin Oryctolagus cuniculus essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations UDP + glucosylated glycogenin
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P13280
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein autoglycosylation Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + glycogenin
-
 Oryctolagus cuniculus UDP + glucosylated glycogenin
-
 ?
UDP-glucose + glycogenin essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations Oryctolagus cuniculus UDP + glucosylated glycogenin
-
 ?
UDP-xylose + glycogenin
-
 Oryctolagus cuniculus UDP + xylosylated glycogenin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, non-glycosylated truncated protein, 0.02 mM enzyme solution Oryctolagus cuniculus
dimer 2 * 38000, native enzyme Oryctolagus cuniculus
monomer 1 * 34000, non-glycosylated truncated protein, 0.0005 mM enzyme solution Oryctolagus cuniculus
monomer 1 * 38000, native enzyme, 50-70% of the activity of the dimer Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
glycogenin
-
 Oryctolagus cuniculus