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Literature summary for 2.4.1.18 extracted from

  • Fan, Q.; Xie, Z.; Zhan, J.; Chen, H.; Tian, Y.
    A glycogen branching enzyme from Thermomonospora curvata: characterization and its action on maize starch (2016), Starch Staerke, 68, 355-364.
No PubMed abstract available
Search for more literature for 2.4.1.18

Cloned(Commentary)

Cloned (Comment) Organism
gene TcGBE, recombinant expression in Escherichia coli strain BL21(DE3) Thermomonospora curvata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, recombinant enzyme, SDS-PAGE Thermomonospora curvata

Organism

Organism UniProt Comment Textmining
Thermomonospora curvata
-
 gene TcGBE
-
Thermomonospora curvata DSM 43183
-
 gene TcGBE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Thermomonospora curvata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90.28
-
 purified recombinnat enzyme, pH 8.5, 55°C, substrate amylose Thermomonospora curvata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information szubstrates are 0.5%, w/v of amylose, corn starch, potato starch, wheat starch and waxy corn starch, all gelatinized by a high temperature (121°C) and pressure (100 KPa) prior treatment. The recombinant enzyme shows the highest specificity to amylose. The recombinant enzyme can act on maize starch, and starch treated with TcGBE is observed to have a lower amylose content and molecular weight. Chain length distribution of amylopectin products by anion exchange chromatography Thermomonospora curvata ?
-
 ?
additional information szubstrates are 0.5%, w/v of amylose, corn starch, potato starch, wheat starch and waxy corn starch, all gelatinized by a high temperature (121°C) and pressure (100 KPa) prior treatment. The recombinant enzyme shows the highest specificity to amylose. The recombinant enzyme can act on maize starch, and starch treated with TcGBE is observed to have a lower amylose content and molecular weight. Chain length distribution of amylopectin products by anion exchange chromatography Thermomonospora curvata DSM 43183 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, recombinant enzyme, SDS-PAGE Thermomonospora curvata

Synonyms

Synonyms Comment Organism
glycogen branching enzyme
-
 Thermomonospora curvata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 recombinant enzyme Thermomonospora curvata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 recombinant enzyme Thermomonospora curvata