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Literature summary for 2.4.1.18 extracted from
- Vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains: N1 domain determines the chain length transferred (2015), FEBS Lett., 589, 1089-1094.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE N1 replacing the counter part of Escherichia coli GBE) mutants. The truncation mutants synthesize branched products with a greatly reduced proportion of short chains compared to the wild-type. The swapping mutant exhibit a branching pattern of the short chain region similar to that of the ewild-type enzyme | Vibrio vulnificus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Vibrio vulnificus | the glycogen branching enzyme from Vibrio vulnificus transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme. The N1-domain of the enzyme has a crucial role in the determination of the branching pattern of glycogen, degrees of polymerization in enzyme reaction products compared to enzymes from other origin, overview | ? | - |
? |  |
| additional information | Vibrio vulnificus MO6-24/O | the glycogen branching enzyme from Vibrio vulnificus transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme. The N1-domain of the enzyme has a crucial role in the determination of the branching pattern of glycogen, degrees of polymerization in enzyme reaction products compared to enzymes from other origin, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio vulnificus | - |
- |
- |
| Vibrio vulnificus MO6-24/O | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the glycogen branching enzyme from Vibrio vulnificus transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme. The N1-domain of the enzyme has a crucial role in the determination of the branching pattern of glycogen, degrees of polymerization in enzyme reaction products compared to enzymes from other origin, overview | Vibrio vulnificus | ? | - |
? | |
| additional information | the glycogen branching enzyme from Vibrio vulnificus transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme. The N1-domain of the enzyme has a crucial role in the determination of the branching pattern of glycogen, degrees of polymerization in enzyme reaction products compared to enzymes from other origin, overview | Vibrio vulnificus MO6-24/O | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GBE | - |
Vibrio vulnificus |
| glycogen branching enzyme | - |
Vibrio vulnificus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Vibrio vulnificus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | activity range, profile overview | Vibrio vulnificus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Vibrio vulnificus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | active at | Vibrio vulnificus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 13, GH13. Phylogenetic analysis groups VvGBE with other Vibrio GBEs and closely related to GBEs of enteric bacteria. VvGBE and Escherichia coli GBE are type I bacterial GBEs and share 57% sequence similarity | Vibrio vulnificus |
| additional information | branching patterns of the truncation mutants and the swapping mutant. Branching patterns of VvGBE wild-type | Vibrio vulnificus |
| physiological function | glycogen is a major polysaccharide of energy reservoir in animals and microorganisms. It is a highly branched polysaccharide, in which glucose residues are linked by alpha-1,4 glycosidic bonds to from linear chains and at every 10 residues, other linear chains are linked by alpha-1,6-glycosidic bonds to form side chains. Formation of side chains in glycogen is catalyzed by glycogen branching enzyme (GBE) or branching enzyme (BE). GBE catalyzes formation of alpha-1,6-glycosidic linkage by cleaving alpha-1,4 linkages of substrate and transferring the non-reducing end of the chain to an acceptor | Vibrio vulnificus |
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Release 2023.1 (January 2023)
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