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Literature summary for 2.4.1.18 extracted from

  • Nakamura, Y.; Utsumi, Y.; Sawada, T.; Aihara, S.; Utsumi, C.; Yoshida, M.; Kitamura, S.
    Characterization of the reactions of starch branching enzymes from rice endosperm (2010), Plant Cell Physiol., 51, 776-794.
    View publication on PubMed
Search for more literature for 2.4.1.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for amylopectin is 3.0 mg/ml for isoform BEI, 2.7 mg/ml for isoform BEIIa, and 0.9 mg/ml for isoform BEIIb, 30°C, pH 7.0 Oryza sativa
0.00018
-
 amylose amylose AS-1160, degree of polymerization of approximately 6,510, isoform BEI, pH 7.5, 30°C Oryza sativa
0.0009
-
 amylose amylose AS-1160, degree of polymerization of approximately 6,510, isoform BEI, pH 7.5, 30°C Oryza sativa
0.0012
-
 amylose amylose AS-1160, degree of polymerization of approximately 6,510, isoform BEI, pH 7.5, 30°C Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin
-
 Oryza sativa amylopectin containing alpha-1,6-glucosidic linkages
-
 ?
amylose
-
 Oryza sativa amylose containing alpha-1,6-glucosidic linkages
-
 ?
additional information The activities of the isozymes BEi, BEIIa and BEIIb with a linear glucan amylose decrease with a decrease in the molar size of amylose, and no activities of BEIIa and BEIIb are found when the degree of polymerization of amylose is lower than at least 80, whereas BEI had an activity with amylose of a degree of polymerization higher than approximately 50. Isoform BEIIb almost exclusively transfers chains with degree of polymerization of 7 and 6 while isoform BEIIa forms a wide range of short chains with degree of polymerization of 6 to around 15 from outer chains of amylopectin and amylose. Isoform BEI forms a variety of short chains and intermediate chains of a degree of polymerization below 40 by attacking not only outer chains but also inner chains of branched glucan. Isoforms BEIIa or BEIIb can only scarcely or can not attack inner chains, respectively Oryza sativa ?
-
 ?