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Literature summary for 2.4.1.18 extracted from
- Heterologous expression and characterization of glycogen branching enzyme from Synechocystis sp. PCC6803 (2008), J. Microbiol. Biotechnol., 18, 1386-1392.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Synechocystis sp. PCC 6803 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90700 | - |
SDS-PAGE and MALDI-TOF-MS analyses | Synechocystis sp. PCC 6803 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechocystis sp. PCC 6803 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3.45fold after Ni-NTA affinity chromatography | Synechocystis sp. PCC 6803 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.29 U/mg for the crude cell extract, 1 U = change in the absorbance of 1 unit | Synechocystis sp. PCC 6803 |
| additional information | - |
1.00 U/mg for the purified enzyme , 1 U = change in the absorbance of 1 unit | Synechocystis sp. PCC 6803 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose | assay at pH 30°C, pH 8.0 | Synechocystis sp. PCC 6803 | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BGE | - |
Synechocystis sp. PCC 6803 |
| glycogen branching enzyme | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Synechocystis sp. PCC 6803 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | no apparent inactivation up to 30°C, about 10% loss of activity at 35°C, about 90% loss of enzyme activity at 50°C | Synechocystis sp. PCC 6803 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Synechocystis sp. PCC 6803 |
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Release 2023.1 (January 2023)
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