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Literature summary for 2.4.1.18 extracted from

  • Devillers, C.H.; Piper, M.E.; Ballicora, M.A.; Preiss, J.
    Characterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus (2003), Arch. Biochem. Biophys., 418, 34-38.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
DELTA1-112 the wild-type enzyme transfers mainly chains with a degree of polymerization of 8-14, the mutant enzyme DELTA1-112 transfers a greater propertion of chains with higher degree of polymerization, 15-20 Escherichia coli
DELTA1-63 the wild-type enzyme transfers mainly chains with a degree of polymerization of 8-14, mutant enzyme has a pattern of transferred chains, 10-20 Escherichia coli
DELTA1-83 the wild-type enzyme transfers mainly chains with a degree of polymerization of 8-14, mutant enzyme has a pattern of transferred chains, 10-20 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and truncation mutants Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.5
-
mutant enzyme DELTA1-63, micromol Glc incorporated into alpha-D-glucan per min at 30°C Escherichia coli
535.4
-
mutant enzyme DELTA1-83, micromol Glc incorporated into alpha-D-glucan per min at 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin the wild-type enzyme transfers mainly chains with a degree of polymerization of 8-14, the mutant enzyme DELTA1-112 transfers a greater propertion of chains with higher degree of polymerization, 15-20. Mutant DELTA1-63 and mutant DELTA1-83 have an intermediate pattern of transferred chains, 10-20. A progressive shortening of the N-terminus leads to a gradual increase in the length of the transferred chains Escherichia coli amylopectin with additional alpha-1,6-glucosidic linkages
-
?
amylose the wild-type enzyme transfers mainly chains with a degree of polymerization of 8-14, the mutant enzyme DELTA1-112 transfers a greater proportion of chains with higher degree of polymerization, 15-20. Mutant DELTA1-63 and mutant DELTA1-83 have an intermediate pattern of transferred chains, 10-20. A progressive shortening of the N-terminus leads to a gradual increase in the length of the transferred chains Escherichia coli amylose containing alpha-1,6-glucosidic linkages
-
?

Synonyms

Synonyms Comment Organism
GBE
-
Escherichia coli