Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y300A | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
Y300D | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
Y300F | mutant enzyme shows 25% of the wild-type activity, no effect on Km-value, heat stability is lowered significantly compared to that of the wild-type enzyme, lower relative activity at elevated temperatures compared to wild-type enzyme | Escherichia coli |
Y300L | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
Y300S | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
Y300W | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
amylose | amylose AS-320, pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
0.0175 | - |
amylose | amylose AS-320, pH 7.0, 30°C, mutant enzyme Y300F | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose | wild-type enzyme and mutant enzyme Y300F both preferentially transfer chains between DP5 and DP16, with a chain of DP11 being transferred at the highest frequency | Escherichia coli | amylose containing alpha-1,6-glucosidic linkages | - |
? |