Protein Variants | Comment | Organism |
---|---|---|
D143A | increase in substrate inhibition with 4-methylumbelliferone, large decrease in Vmax | Homo sapiens |
D148A | large decrease in Vmax | Homo sapiens |
H37A | H37 is not critical in N-glucuronidation, but in O-glucuronidation. 99% decrease in Vmax with substrate UDP-glucuronate | Homo sapiens |
H37D | H37 is not critical in N-glucuronidation, but in O-glucuronidation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Methylumbelliferone | substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
4-Methylumbelliferone | mutant D143A | Homo sapiens | |
0.021 | - |
4-Methylumbelliferone | wild-type | Homo sapiens | |
0.058 | - |
retigabine | mutant H37A | Homo sapiens | |
0.062 | - |
scopoletin | mutant D143A | Homo sapiens | |
0.083 | - |
scopoletin | wild-type | Homo sapiens | |
0.09 | - |
retigabine | wild-type | Homo sapiens | |
0.138 | - |
UDP-glucuronate | mutant D143A | Homo sapiens | |
0.173 | - |
UDP-glucuronate | mutant H37A | Homo sapiens | |
0.179 | - |
UDP-glucuronate | wild-type | Homo sapiens | |
0.184 | - |
UDP-glucuronate | mutant D148A | Homo sapiens | |
0.251 | - |
4-aminobiphenyl | mutant D148A | Homo sapiens | |
0.269 | - |
4-aminobiphenyl | wild-type | Homo sapiens | |
0.286 | - |
4-aminobiphenyl | mutant H37A | Homo sapiens | |
0.629 | - |
scopoletin | mutant D148A | Homo sapiens | |
0.954 | - |
scopoletin | mutant H37A | Homo sapiens | |
0.967 | - |
4-Methylumbelliferone | mutant D148A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform UGT1A9 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobiphenyl + UDP-glucuronate | - |
Homo sapiens | UDP + 4-aminobiphenyl N-glucuronide | - |
? | |
4-methylumbelliferone + UDP-glucuronate | - |
Homo sapiens | UDP + 4-methylumbelliferyl-7-O-beta-D-glucuronide | - |
? | |
retigabine + UDP-glucuronate | - |
Homo sapiens | UDP + retigabine N-glucuronide | - |
? | |
scopoletin + UDP-glucuronate | - |
Homo sapiens | UDP + scopoletin O-glucuronide | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-Methylumbelliferone | mutant D143A | Homo sapiens | |
3.73 | - |
4-Methylumbelliferone | wild-type | Homo sapiens |