General Stability | Organism |
---|---|
phosphatidylcholine stabilizes the detergent treated enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | markedly reduces 4-methylumbelliferone UGT activity only when detergent-treated rat liver microsomes are used as the enzyme source, inhibits UGT activity non-competitively without competing with either 4-methylumbelliferone or UDP-D-glucuronic acid | Rattus norvegicus | |
AMP | inhibits exclusively the estradiol 17-glucuronidation activity | Rattus norvegicus | |
ATP | markedly reduces 4-methylumbelliferone UGT activity only when detergent-treated rat liver microsomes are used as the enzyme source, inhibits UGT activity non-competitively without competing with either 4-methylumbelliferone or UDP-D-glucuronic acid | Rattus norvegicus | |
CTP | no inhibition of UGT by other cytosine-related nucleotides but CTP | Rattus norvegicus | |
GTP | weak inhibition, no inhibition of UGT by other guanosine-related nucleotides but GTP | Rattus norvegicus | |
additional information | AMP antagonizes the inhibitory effects of adenine, ATP, and NADP+ on 4-methylumbelliferone and estradiol 3-glucuronidation activities, no inhibition by glucose 1-phosphate, glucose 6-phosphate, fructose 1-phosphate, and fructose 6-phosphate, and by cytidine, dCTP, cytosine, CMP, dGTP, guanosine, GDP, GMP, NADH, NADPH, dATP, and adenosine | Rattus norvegicus | |
NAD+ | inhibits UGT activity non-competitively without competing with either 4-methylumbelliferone or UDP-D-glucuronic acid | Rattus norvegicus | |
NADP+ | markedly reduces 4-methylumbelliferone UGT activity only when detergent-treated rat liver microsomes are used as the enzyme source, inhibits UGT activity non-competitively without competing with either 4-methylumbelliferone or UDP-D-glucuronic acid | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Rattus norvegicus | 5789 | - |
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate + acceptor | Rattus norvegicus | - |
UDP + acceptor beta-D-glucuronoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by microsome preparation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate + 4-methylumbelliferone | - |
Rattus norvegicus | UDP + 4-methylumbelliferyl beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + acceptor | - |
Rattus norvegicus | UDP + acceptor beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + estradiol | - |
Rattus norvegicus | UDP + estradiol-3-beta-D-glucuronoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase | - |
Rattus norvegicus |
UGT | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0059 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | CTP | |
0.008 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | NADP+ | |
0.009 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | NAD+ | |
0.01 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | NADP+ | |
0.0117 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | ATP | |
0.0118 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | NAD+ | |
0.0154 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | ATP | |
0.0252 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | NADP+ | |
0.0259 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | ADP | |
0.0272 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | ADP | |
0.031 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | AMP | |
0.0321 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | NAD+ | |
0.0347 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | CTP | |
0.0352 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | ATP | |
0.0558 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | CTP | |
0.0774 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | ADP | |
0.108 | - |
pH 7.1, 37°C, inhibition of 4-methylumbelliferone glucuronidation activity | Rattus norvegicus | GTP | |
0.129 | - |
pH 7.1, 37°C, inhibition of estradiol 17-glucuronidation activity | Rattus norvegicus | GTP | |
0.227 | - |
pH 7.1, 37°C, inhibition of estradiol 3-glucuronidation activity | Rattus norvegicus | GTP |