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Literature summary for 2.4.1.17 extracted from
- Steroidglucuronyltransferases. 3. Oestradiol-17 3-glucuronyltransferase of the mitochondria of pig intestine (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 1784-1788.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | 10 mM, slight activation | Sus scrofa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | inhibitor at 10 mM, no effect below | Sus scrofa | |
| diclofenac | - |
Sus scrofa | |
| estrone | competitive to estradiol-17beta | Sus scrofa | |
| UDP | 0.05 mM inhibits 60% of the activity of the mitochondrial enzyme | Sus scrofa | |
| Zn2+ | inhibitor above 10mM | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
17beta-estradiol | - |
Sus scrofa | |
| 0.046 | - |
estradiol-17beta | range between 0.045 mM and 0.047 mM | Sus scrofa | |
| 0.16 | - |
UDPglucuronic acid | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sus scrofa | 5829 | - |
| microsome | - |
Sus scrofa | - |
- |
| mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20 mM, activation | Sus scrofa | |
| Mg2+ | activation at 20 mM | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
pig | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| small intestine | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| estradiol-17beta + UDPglucuronate | - |
Sus scrofa | estradiol-17beta-3-monoglucuronide + UDP | - |
? | |
| estriol + UDPglucuronate | - |
Sus scrofa | UDP + estriol-16 alpha-glucuronide | - |
? | |
| estrone + UDPglucuronate | - |
Sus scrofa | UDP + estrone 3-O-glucuronide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
activity increases with rise in incubation temperature | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivation in absence of substrates | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
Tris/HCl buffer, in Tris/maleate and phosphate buffers the enzyme shows this pH optimum but lower activity | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
diclofenac | - |
Sus scrofa | |
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