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Literature summary for 2.4.1.17 extracted from
- Purification of properties of rabbit liver estrone and p-nitrophenol UDP-glucuronyltransferases (1981), Arch. Biochem. Biophys., 209, 565-578.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
estrone | - |
Oryctolagus cuniculus |  |
| 1.5 | - |
4-nitrophenol | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000 | Oryctolagus cuniculus |
| 230000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
rabbit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, chromatography on DEAE-cellulose and affinity chromatography on UDP-hexanolamine sepharose | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, half-life 18-20 days | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenol + UDP-glucuronate | - |
Oryctolagus cuniculus | 4-nitrophenyl beta-D-glucuronide + UDP | - |
? | |
| estrone + UDPglucuronate | estrone-specific activity inactive towards 4-nitrophenol | Oryctolagus cuniculus | UDP + estrone 3-O-glucuronide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000 | Oryctolagus cuniculus |
| ? | all values determined by SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
4-nitrophenol | Oryctolagus cuniculus |
| 8 | 9 | estrone | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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