Literature summary for 2.4.1.16 extracted from

Gay, L.; Hebraud, M.; Girard, V.; Fevre, M.
Chitin synthase activity from Neocallimastix frontalis, an anaerobic rumen fungus (1989), J. Gen. Microbiol., 135, 279-283.

No PubMed abstract available

Search for more literature for 2.4.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
GlcNAc	20 mM stimulates, allosteric activator	Neocallimastix frontalis

Inhibitors

Inhibitors	Comment	Organism	Structure
Polyoxin D	-	Neocallimastix frontalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	UDP-GlcNAc	-	Neocallimastix frontalis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Neocallimastix frontalis	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	divalent cation required for maximal activity, Mg2+ is most efficient	Neocallimastix frontalis

Organism

Organism	UniProt	Comment	Textmining
Neocallimastix frontalis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	following treatment with trypsin, the chitin synthase activity is increased by 6fold, indicating that most of the chitin synthase activity is zymogenic	Neocallimastix frontalis

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Neocallimastix frontalis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n	-	Neocallimastix frontalis	UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1	chitin	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
32	-	-	Neocallimastix frontalis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	10 mM Tris/HCl buffer	Neocallimastix frontalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.004	-	Polyoxin D	-	Neocallimastix frontalis

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Release 2023.1 (January 2023)