Cloned (Comment) | Organism |
---|---|
gene tps1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Ascaris suum |
gene tps2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Ascaris suum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 55% inhibition at 10 mM; 55% inhibition at 10 mM | Ascaris suum | |
Fe3+ | 32% inhibition at 10 mM; 32% inhibition at 10 mM | Ascaris suum | |
K+ | 50% inhibition at 10 mM; 50% inhibition at 10 mM | Ascaris suum | |
Zn2+ | - |
Ascaris suum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | activates 1.44fold at 10 mM | Ascaris suum | |
MgCl2 | activates 2fold at 10 mM | Ascaris suum | |
NaCl | activates at 10 mM | Ascaris suum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Ascaris suum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | H2BIV4 | gene tps1 | - |
Ascaris suum | H2BIV5 | gene TPS2 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from female worm muscle 265fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Ascaris suum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Ascaris suum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150.5 | - |
purified native enzyme, pH 4.0, 37°C | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the purified native enzyme is not absolutely substrate-specific, besides glucose 6-phosphate, the enzyme is able to use fructose 6-phosphate as an acceptor of glucose. The enzyme is inactive in the presence of glucose and fructose | Ascaris suum | ? | - |
? | |
UDP-glucose + D-glucose 6-phosphate | - |
Ascaris suum | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Ascaris suum |
Synonyms | Comment | Organism |
---|---|---|
T6P synthase | - |
Ascaris suum |
TPS | - |
Ascaris suum |
trehalose 6-phosphate synthase | - |
Ascaris suum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Ascaris suum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, at 40°C, the enzyme demonstrates 87.04% of its maximum activity. A rapid decrease in the enzyme activity occurs at 50°C with 35.27% of maximum activity, completely loss of activity above 60°C | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | 4.2 | - |
Ascaris suum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | activity range | Ascaris suum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ascaris suum | isoelectric focusing | - |
5.4 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glucosyltransferase-GTB-type superfamily | Ascaris suum |