Protein Variants | Comment | Organism |
---|---|---|
D156G | site-directed mutagenesis of a catalytic site residue, catalytically inactive Tps1 mutant variant | Saccharomyces cerevisiae |
additional information | construction of a tps1DELTA and a tps1-156 deletion mutant | Saccharomyces cerevisiae |
W111S | site-directed mutagenesis of a catalytic site residue | Saccharomyces cerevisiae |
Y102V | site-directed mutagenesis of a catalytic site residue | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-glucose 6-phosphate | Saccharomyces cerevisiae | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q00764 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-glucose 6-phosphate | - |
Saccharomyces cerevisiae | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS1 | - |
Saccharomyces cerevisiae |
trehalose-6P synthase | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | stress hypersensitivity of mutants deleted for gene TPS1. At 42°C, the viability of the tps1DELTA mutant sharply drops by several decades, whereas the viability of the wild-type TPS1 strain remains almost unchanged. The the mal- BY4741 strain is unable to import trehalose. Overview of the impact of the different stresses on the drop of viability in wild-type and mdeletion mutants. The tps1DELTA mutant rapidly loses its ATP content in response to heat shock at 42°C | Saccharomyces cerevisiae |
metabolism | gene TPS1 encodes the first enzyme in trehalose biosynthetic pathway, trehalose is a stress protectant | Saccharomyces cerevisiae |
physiological function | the Tps1 protein is essential to maintain ATP levels during heat shock. Tps1 is endowed with a regulatory function in energy homeostasis, which is essential to withstand adverse conditions and maintain cellular integrity. Tps1, not trehalose, is important for yeast viability in response to various kinds of stresses. Contribution of the Tps1 protein to thermotolerance and acquired thermotolerance, enzyme deletion mutants show highly reduced growth at 42°C in contrast to the wild-type enzyme | Saccharomyces cerevisiae |