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Literature summary for 2.4.1.15 extracted from
- Trehalose phosphate synthases OtsA1 and OtsA2 of Rhodococcus opacus 1CP (2013), FEMS Microbiol. Lett., 342, 113-122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| heparin | the activity of OtsA2-His10 is significantly increased, most of all when UDP-glucose is used as substrate | Rhodococcus opacus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene otsA1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression as His10-tagged isozyme in Escherichia coli strain BL21 CodonPlus(DE3)-RP | Rhodococcus opacus |
| gene otsA2, DNA and amino acid sequence determination and analysis, , phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression as His10-tagged isozyme in Escherichia coli strain BL21 CodonPlus(DE3)-RP | Rhodococcus opacus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | heparin in assays has no effect on the activity of recombinant OtsA1-His10 | Rhodococcus opacus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + D-glucose 6-phosphate | Rhodococcus opacus | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | Rhodococcus opacus 1CP | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus opacus | A4K8Z1 | OtsA1 | - |
| Rhodococcus opacus | A4K8Z2 | OtsA2 | - |
| Rhodococcus opacus 1CP | A4K8Z1 | OtsA1 | - |
| Rhodococcus opacus 1CP | A4K8Z2 | OtsA2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His10-tagged isozyme from Escherichia coli strain BL21 CodonPlus(DE3)-RP by nickel affinity chromatography, ultrafiltration, tag cleavage by Factor Xa | Rhodococcus opacus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, recombinant His10-tagged isozyme OtsA2 gel filtration pool for 7 days yields 76% of its initial activity | Rhodococcus opacus |
| -20°C, recombinant His10-tagged isozyme OtsA2 gel filtration pool loses its activity after thawing | Rhodococcus opacus |
| 4°C, purified recombinant His10-tagged isozyme OtsA1almost loses its entire activity overnight | Rhodococcus opacus |
| 4°C, purified recombinant His10-tagged isozyme OtsA2 keeps a stable activity over several days | Rhodococcus opacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + D-glucose 6-phosphate | recombinant His10-tagged OtsA1 shows highest activity with ADP-glucose and UDP-glucose, whereas recombinant His10-tagged OtsA2 prefers UDP-glucose | Rhodococcus opacus | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| ADP-glucose + D-glucose 6-phosphate | recombinant His10-tagged OtsA1 shows highest activity with ADP-glucose and UDP-glucose, whereas recombinant His10-tagged OtsA2 prefers UDP-glucose | Rhodococcus opacus 1CP | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | - |
Rhodococcus opacus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | recombinant His10-tagged OtsA1 shows highest activity with ADP-glucose and UDP-glucose, whereas recombinant His10-tagged OtsA2 prefers UDP-glucose | Rhodococcus opacus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | - |
Rhodococcus opacus 1CP | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | recombinant His10-tagged OtsA1 shows highest activity with ADP-glucose and UDP-glucose, whereas recombinant His10-tagged OtsA2 prefers UDP-glucose | Rhodococcus opacus 1CP | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OtsA1 | - |
Rhodococcus opacus |
| OtsA2 | - |
Rhodococcus opacus |
| trehalose phosphate synthase | - |
Rhodococcus opacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Rhodococcus opacus |
| 37 | - |
assay at | Rhodococcus opacus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | drastic decrease in enzyme activity is observed at temperatures below 20°C and above 50°C | Rhodococcus opacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodococcus opacus |
| 8 | - |
assay at | Rhodococcus opacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | drastic decrease in enzyme activity is observed at pH values below pH 6.0 and above pH 8.0 | Rhodococcus opacus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | in the OtsAB pathway, a trehalose-6-phosphate synthase (TPS, OtsA) catalyzes the formation of trehalose-6-phosphate from a glucosyl nucleotide and glucose-6-phosphate | Rhodococcus opacus |
| physiological function | isozyme OstA2 mainly contributes to the trehalose pool of strain 1CP | Rhodococcus opacus |
| physiological function | isozyme OtsA1 seems to be involved in the overproduction of trehalose lipids | Rhodococcus opacus |
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