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Literature summary for 2.4.1.15 extracted from

  • Jiang, Y.; Chen, X.M.; Liu, Y.J.; Li, Y.T.; Zhang, H.H.; Dyson, P.; Sheng, H.M.; An, L.Z.
    The catalytic efficiency of trehalose-6-phosphate synthase is effected by the N-loop at low temperatures (2010), Arch. Microbiol., 192, 937-943.
    View publication on PubMed
Search for more literature for 2.4.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene otsA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21(DE3) Arthrobacter sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate Arthrobacter sp.
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate Arthrobacter sp. Cjts
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q1KLW0 gene otsA
-
Arthrobacter sp. Cjts Q1KLW0 gene otsA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B21(DE3) by nickel affinity chromatography Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Arthrobacter sp. UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Arthrobacter sp. Cjts UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
OtsA
-
 Arthrobacter sp.
trehalose-6-phosphate synthase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 37 tested activity range Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arthrobacter sp.

General Information

General Information Comment Organism
physiological function the multifunctional protein, OtsA, is indispensable for bacterial survival in various environments. The catalytic efficiency of OtsA is affected by its N-loop at low temperatures. The flexibility of the OtsA N-loop is related to the growth temperature of the respective bacterial species Arthrobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures Arthrobacter sp.