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Literature summary for 2.4.1.15 extracted from

  • Miranda, J.A.; Avonce, N.; Suarez, R.; Thevelein, J.M.; Van Dijck, P.; Iturriaga, G.
    A bifunctional TPS-TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis (2007), Planta, 226, 1411-1421.
    View publication on PubMed
Search for more literature for 2.4.1.15

Application

Application Comment Organism
agriculture the results demonstrate that engineering trehalose metabolism with a yeast TPS-TPP bifunctional enzyme confers multiple stress protection in plants, comprising a potential tool to improve stress-tolerance in crops Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
a translational trehalose-6-phosphate synthase - trehalose-6-phosphate phosphatase, TPS-TPP, gene-fusion is constructed and subcloned into the pBluescript SK vector, into the yeast expression vector pSal4, and into the pBin19 vector for transformation of Arabidopsis thaliana Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate Saccharomyces cerevisiae
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4 Saccharomyces cerevisiae
0.013
-
 TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 Saccharomyces cerevisiae
0.02
-
 TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 Saccharomyces cerevisiae
0.055
-
 TPS specific activity in galactose, yeast wild type + pSAL4 Saccharomyces cerevisiae
0.063
-
 TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS2 Saccharomyces cerevisiae
0.067
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS2 Saccharomyces cerevisiae
0.073
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.078
-
 TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.079
-
 TPS specific activity in glucose, yeast wild type + pSAL4 Saccharomyces cerevisiae
0.081
-
 TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.086
-
 TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.088
-
 TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.093
-
 TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
0.144
-
 TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1 Saccharomyces cerevisiae
0.163
-
 TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
TPS1
-
 Saccharomyces cerevisiae
trehalose-6-phosphate synthase
-
 Saccharomyces cerevisiae