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Literature summary for 2.4.1.15 extracted from
- Trehalose biosynthesis in Thermus thermophilus RQ-1: biochemical properties of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase (2005), Extremophiles, 9, 29-36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene otsA, cloned from genetic library, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
UDP-glucose | recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+ | Thermus thermophilus |  |
| 0.8 | - |
D-glucose 6-phosphate | recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+ | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Thermus thermophilus | |
| Mg2+ | stimulates | Thermus thermophilus | |
| additional information | no requirement for divalent cations for activity | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate | Thermus thermophilus | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | Thermus thermophilus RQ-1 | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q7WUI7 | gene otsA | - |
| Thermus thermophilus RQ-1 | Q7WUI7 | gene otsA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.6 | - |
purified recombinant enzyme, substrates GDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
| 95 | - |
purified recombinant enzyme, substrates ADP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
| 106.7 | - |
purified recombinant enzyme, substrates TDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
| 149.6 | - |
purified recombinant enzyme, substrates UDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| ADP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus RQ-1 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-alpha-D-glucose + D-glucose 6-phosphate | much less effective than UDP-glucose | Thermus thermophilus | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-alpha-D-glucose + D-glucose 6-phosphate | much less effective than UDP-glucose | Thermus thermophilus RQ-1 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| additional information | donor substrate specificity in descending order: UDP-glucose, TDP-glucose, ADP-glucose, GDP-glucose, no activity with mannose-6-phosphate, fructose 6-phosphate, or glucose 1,6-diphosphate as acceptors | Thermus thermophilus | ? | - |
? | |
| additional information | donor substrate specificity in descending order: UDP-glucose, TDP-glucose, ADP-glucose, GDP-glucose, no activity with mannose-6-phosphate, fructose 6-phosphate, or glucose 1,6-diphosphate as acceptors | Thermus thermophilus RQ-1 | ? | - |
? | |
| TDP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus | TDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | Thermus thermophilus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | best substrates, absolutely specific for D-glucose 6-phosphate | Thermus thermophilus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | Thermus thermophilus RQ-1 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | best substrates, absolutely specific for D-glucose 6-phosphate | Thermus thermophilus RQ-1 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPS | - |
Thermus thermophilus |
| trehalose-6-phosphate synthase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 98 | - |
- |
Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | about half-maximal activity at 40°C, 80% of maximal activity at 100°C, inactive at 30°C | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 193 min | Thermus thermophilus |
| 90 | - |
half-life: 54 min | Thermus thermophilus |
| 100 | - |
half-life: 31 min | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermus thermophilus |
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