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Literature summary for 2.4.1.131 extracted from

  • Schutzbach, J.S.; Springfield, J.D.; Jensen, J.W.
    The biosynthesis of oligosaccharide-lipids. Formation of an alpha-1,2-mannosyl-mannose linkage (1980), J. Biol. Chem., 255, 4170-4175.
    View publication on PubMed
Search for more literature for 2.4.1.131

Inhibitors

Inhibitors Comment Organism Structure
ADP 2 mM, less than 15% inhibition Oryctolagus cuniculus
ATP 2 mM, less than 15% inhibition Oryctolagus cuniculus
deoxycholate
-
 Oryctolagus cuniculus
GDP 0.02 mM, 84% inhibition; 2 mM, 84% inhibition Oryctolagus cuniculus
GMP 0.02 mM, 30% inhibition; 2 mM, 30% inhibition Oryctolagus cuniculus
GTP 0.02 mM, 40% inhibition; 2 mM, 40% inhibition Oryctolagus cuniculus
additional information less than 15% inhibition at 2 mM: ADP, ATP, UDP, UDP-glucose, UDP-galactose and UDP-N-acetylglucosamine; not inhibited by EDTA Oryctolagus cuniculus
taurocholate
-
 Oryctolagus cuniculus
UDP 2 mM, less than 15% inhibition Oryctolagus cuniculus
UDP-galactose 2 mM, less than 15% inhibition Oryctolagus cuniculus
UDP-glucose 2 mM, less than 15% inhibition Oryctolagus cuniculus
UDP-N-acetylglucosamine 2 mM, less than 15% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00064
-
 D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol pH 7.0, 37°C Oryctolagus cuniculus
0.00064
-
 oligosaccharide-lipid acceptor based on the assumption of incorporation of only a single mannosyl unit into the product Oryctolagus cuniculus
0.0047
-
 GDPmannose
-
 Oryctolagus cuniculus
0.0047
-
 GDP-D-mannose pH 7.0, 37°C Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Oryctolagus cuniculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement of divalent cations Oryctolagus cuniculus
additional information reaction is independent of the addition of divalent cations Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDPmannose + oligosaccharide-lipid acceptor Oryctolagus cuniculus biosynthesis of oligosaccharide-lipids GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Oryctolagus cuniculus
-
 New Zealand white rabbits
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus
solubilized with 0.15% v/v Nonidet P-40, partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
0-4°C, solubilized enzyme, at least 2 weeks, stable Oryctolagus cuniculus
0°C, partially purified enzyme, 5 days, stable Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 Oryctolagus cuniculus 2 UDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 ?
GDP-mannose + oligosaccharide-lipid acceptor acceptor isolated from pig liver Oryctolagus cuniculus GDP + alpha-mannosyl-1,2-oligosaccharide-lipid presumably a heptasaccharide with a 1,6-linked branched glycosyl unit with 65% of the newly added mannosyl units in a terminal nonreducing position, structure may be Manalpha(1-2)Man-Man(Man(1-6))Man-GlcNAc-GlcNAc ?
GDPmannose + oligosaccharide-lipid acceptor biosynthesis of oligosaccharide-lipids Oryctolagus cuniculus GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
 ?
additional information no substrate: dolichol-phosphat Oryctolagus cuniculus additional information
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Oryctolagus cuniculus