Cloned (Comment) | Organism |
---|---|
sus3, recombinant expression of wild-type and mutant enzymes in Pichia pastoris | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
E678D | site-directed mutagenesis, almost inactive mutant | Oryza sativa |
E678Q | site-directed mutagenesis, almost inactive mutant, the mutant shows impaired D-fructose binding | Oryza sativa |
E686D | site-directed mutagenesis, the mutant enzyme retains 34.9% of sucrose cleavage and 37.9% of sucrose synthesis activity, respectively | Oryza sativa |
E686Q | site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding | Oryza sativa |
F680S | site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding | Oryza sativa |
F680Y | site-directed mutagenesis, the mutant enzyme retains 61.0% of sucrose cleavage and all of sucrose synthesis activity, respectively | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required in sucrose synthesis reaction | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
336000 | 375000 | recombinant wild-type and mutant enzymes, gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose | Oryza sativa | preferred substrates in both reaction directions | UDP + sucrose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q43009 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Pichia pastris by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose | preferred substrates in both reaction directions | Oryza sativa | UDP + sucrose | - |
r | |
UDP-glucose + D-fructose | the binding of beta-fructopyranose involves residues from both the GT-BN and the GT-BC domains, including those in the E-X7-E motif | Oryza sativa | UDP + sucrose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
RSuS3 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
sucrose cleavage | Oryza sativa |
7.5 | - |
sucrose synthesis | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to family 4 of the glycosyltransferases (GT4) and contains an E-X7-E motif that is conserved in members of GT4 and two other GT families, sequence comparisons, overview | Oryza sativa |
metabolism | key enzyme in sucrose metabolism in higher plants | Oryza sativa |
additional information | enzyme homology modeling and substrate docking using the crystal structure of Nitrosomonas europaea NeSuS1, PDB ID 4RBN, and the crystal structure of Arabidopsis AtSuS1, PDB ID 3S28, overview | Oryza sativa |
physiological function | the enzyme activity is finely tuned through regulation of their gene expression at different levels and modulation of enzymatic activities by metabolites | Oryza sativa |