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Literature summary for 2.4.1.13 extracted from
- Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes (2015), Appl. Microbiol. Biotechnol., 99, 8465-8474.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SuSyAc, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acidithiobacillus caldus |
| gene SuSyDa, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Denitrovibrio acetiphilus |
| gene SuSyMr, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Melioribacter roseus |
| gene SuSyNe, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Nitrosomonas europaea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A642N | site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme | Acidithiobacillus caldus |
| L636Q/A642N | site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme | Acidithiobacillus caldus |
| L636Q/V641R/A642N | site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme | Acidithiobacillus caldus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | substrate inhibition occurs in the presence of GDP at concentrations above 10 mM | Nitrosomonas europaea |  |
| Mg2+ | - |
Denitrovibrio acetiphilus | |
| Mg2+ | - |
Melioribacter roseus | |
| Mg2+ | - |
Nitrosomonas europaea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Denitrovibrio acetiphilus | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Melioribacter roseus | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Acidithiobacillus caldus | |
| additional information | - |
additional information | Michaelis-Menten kinetics, except for GDP | Nitrosomonas europaea | |
| 0.17 | - |
ADP | pH 7.0, 60°C | Acidithiobacillus caldus | |
| 0.44 | - |
ADP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 0.69 | - |
UDP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 1.28 | - |
CDP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 1.56 | - |
GDP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 7.8 | - |
UDP | pH 7.0, 60°C | Acidithiobacillus caldus | |
| 8.5 | - |
GDP | pH 7.0, 60°C | Acidithiobacillus caldus | |
| 16.9 | - |
CDP | pH 7.0, 60°C | Acidithiobacillus caldus | |
| 140 | - |
sucrose | pH 7.0, 60°C, with ADP | Nitrosomonas europaea | |
| 321 | - |
sucrose | pH 7.0, 60°C, with UDP | Nitrosomonas europaea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slightly stimulating | Acidithiobacillus caldus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NDP-glucose + D-fructose | Denitrovibrio acetiphilus | - |
NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | Melioribacter roseus | - |
NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | Nitrosomonas europaea | - |
NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | Acidithiobacillus caldus | - |
NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | Denitrovibrio acetiphilus DSM 12809 | - |
NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | Melioribacter roseus JCM 17771 | - |
NDP + sucrose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus caldus | A0A059ZV61 | - |
- |
| Acidithiobacillus caldus ATCC 51756 | A0A059ZV61 | - |
- |
| Denitrovibrio acetiphilus | D4H6M0 | - |
- |
| Denitrovibrio acetiphilus DSM 12809 | D4H6M0 | - |
- |
| Melioribacter roseus | I7A3T6 | - |
- |
| Melioribacter roseus JCM 17771 | I7A3T6 | - |
- |
| Nitrosomonas europaea | Q820M5 | - |
- |
| Nitrosomonas europaea ATCC 19718 | Q820M5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Denitrovibrio acetiphilus |
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Melioribacter roseus |
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Nitrosomonas europaea |
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Acidithiobacillus caldus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal growth at 20-30°C | Nitrosomonas europaea | - |
| additional information | optimal growth at 35-37°C | Denitrovibrio acetiphilus | - |
| additional information | optimal growth at 45°C | Acidithiobacillus caldus | - |
| additional information | optimal growth at 52-55°C | Melioribacter roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + D-fructose | - |
Denitrovibrio acetiphilus | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Melioribacter roseus | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Nitrosomonas europaea | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Acidithiobacillus caldus | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Nitrosomonas europaea ATCC 19718 | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Denitrovibrio acetiphilus DSM 12809 | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Melioribacter roseus JCM 17771 | ADP + sucrose | - |
r | |
| ADP-glucose + D-fructose | - |
Acidithiobacillus caldus ATCC 51756 | ADP + sucrose | - |
r | |
| CDP-glucose + D-fructose | - |
Nitrosomonas europaea | CDP + sucrose | - |
r | |
| CDP-glucose + D-fructose | - |
Acidithiobacillus caldus | CDP + sucrose | - |
r | |
| CDP-glucose + D-fructose | - |
Nitrosomonas europaea ATCC 19718 | CDP + sucrose | - |
r | |
| CDP-glucose + D-fructose | - |
Acidithiobacillus caldus ATCC 51756 | CDP + sucrose | - |
r | |
| GDP-glucose + D-fructose | - |
Nitrosomonas europaea | GDP + sucrose | - |
r | |
| GDP-glucose + D-fructose | - |
Acidithiobacillus caldus | GDP + sucrose | - |
r | |
| GDP-glucose + D-fructose | - |
Nitrosomonas europaea ATCC 19718 | GDP + sucrose | - |
r | |
| GDP-glucose + D-fructose | - |
Acidithiobacillus caldus ATCC 51756 | GDP + sucrose | - |
r | |
| additional information | UDP-glucose is preferred before ADP-glucose | Denitrovibrio acetiphilus | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Melioribacter roseus | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Nitrosomonas europaea | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Acidithiobacillus caldus | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Nitrosomonas europaea ATCC 19718 | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Denitrovibrio acetiphilus DSM 12809 | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Melioribacter roseus JCM 17771 | ? | - |
? | |
| additional information | UDP-glucose is preferred before ADP-glucose | Acidithiobacillus caldus ATCC 51756 | ? | - |
? | |
| NDP-glucose + D-fructose | - |
Denitrovibrio acetiphilus | NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | - |
Melioribacter roseus | NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | - |
Nitrosomonas europaea | NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | - |
Acidithiobacillus caldus | NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | - |
Denitrovibrio acetiphilus DSM 12809 | NDP + sucrose | - |
r | |
| NDP-glucose + D-fructose | - |
Melioribacter roseus JCM 17771 | NDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Denitrovibrio acetiphilus | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Melioribacter roseus | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Nitrosomonas europaea | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Acidithiobacillus caldus | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Nitrosomonas europaea ATCC 19718 | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Denitrovibrio acetiphilus DSM 12809 | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Melioribacter roseus JCM 17771 | UDP + sucrose | - |
r | |
| UDP-glucose + D-fructose | - |
Acidithiobacillus caldus ATCC 51756 | UDP + sucrose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure-function relationship analysis, overview | Denitrovibrio acetiphilus |
| More | structure-function relationship analysis, overview | Melioribacter roseus |
| More | structure-function relationship analysis, overview | Nitrosomonas europaea |
| More | structure-function relationship analysis, overview | Acidithiobacillus caldus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SuSy | - |
Denitrovibrio acetiphilus |
| SuSy | - |
Melioribacter roseus |
| SuSy | - |
Nitrosomonas europaea |
| SuSy | - |
Acidithiobacillus caldus |
| SuSyAc | - |
Acidithiobacillus caldus |
| SuSyDa | - |
Denitrovibrio acetiphilus |
| SuSyMr | - |
Melioribacter roseus |
| SuSyNe | - |
Nitrosomonas europaea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acidithiobacillus caldus |
| 65 | - |
- |
Denitrovibrio acetiphilus |
| 75 | - |
- |
Nitrosomonas europaea |
| 80 | - |
- |
Melioribacter roseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant His-tagged enzyme, without substrates, 15 min, 38% activity remaining | Melioribacter roseus |
| 60 | - |
purified recombinant His-tagged enzyme, without substrates, 15 min, 54% activity remaining | Nitrosomonas europaea |
| 60 | - |
purified recombinant His-tagged enzyme, without substrates, 15 min, 96% activity remaining | Acidithiobacillus caldus |
| 60 | - |
purified recombinant His-tagged enzyme, without substrates, 15 min, no activity remaining | Denitrovibrio acetiphilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Nitrosomonas europaea |
| 5.5 | - |
- |
Acidithiobacillus caldus |
| 6 | - |
- |
Denitrovibrio acetiphilus |
| 7 | - |
- |
Melioribacter roseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | over 40% of maximal enzyme activity within this range | Denitrovibrio acetiphilus |
| 5.5 | 7.5 | over 40% of maximal enzyme activity within this range | Melioribacter roseus |
| 5.5 | 7.5 | over 40% of maximal enzyme activity within this range | Nitrosomonas europaea |
| 5.5 | 7.5 | over 40% of maximal enzyme activity within this range | Acidithiobacillus caldus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
GDP | pH 7.0, 60°C | Nitrosomonas europaea | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structure-function relationship analysis, overview | Denitrovibrio acetiphilus |
| additional information | structure-function relationship analysis, overview | Melioribacter roseus |
| additional information | structure-function relationship analysis, overview | Nitrosomonas europaea |
| additional information | structure-function relationship analysis, overview | Acidithiobacillus caldus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
sucrose | pH 7.0, 60°C, with UDP | Nitrosomonas europaea | |
| 1 | - |
sucrose | pH 7.0, 60°C, with ADP | Nitrosomonas europaea | |
| 14 | - |
CDP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 39 | - |
GDP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 71 | - |
ADP | pH 7.0, 60°C | Nitrosomonas europaea | |
| 150 | - |
UDP | pH 7.0, 60°C | Nitrosomonas europaea | |
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