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Literature summary for 2.4.1.13 extracted from
- Broadening the biocatalytic properties of recombinant sucrose synthase 1 from potato (Solanum tuberosum L.) by expression in Escherichia coli and Saccharomyces cerevisiae (2008), Enzyme Microb. Technol., 43, 289-296.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sus1, expression of wild-type enzyme and mutant S11D in Escherichia coli strain BL21(DE3) reveals monosaccharides D-ribulose, D-tagatose, L-glucose, and L-rhamnose as additional acceptor substrates, expression of wild-type SuSy1 and SuSy1 S11A mutant in Saccharomyces cerevisiae strain BY4741 with phosphorylation of the wild-type enzyme at Ser11 | Solanum tuberosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S11A | site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Saccharomyces cerevisiae | Solanum tuberosum |
| S11D | site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Escherichia coli | Solanum tuberosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine | 30% inhibition | Solanum tuberosum |  |
| 5-deoxy-D-fructose | 10% inhibition | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
UDP | recombinant enzyme expressed in Escherichia coli | Solanum tuberosum | |
| 0.3 | - |
UDP | recombinant enzyme expressed in Saccharomyces cerevisiae | Solanum tuberosum | |
| 0.6 | - |
UDP-alpha-D-glucose | recombinant enzyme expressed in Saccharomyces cerevisiae | Solanum tuberosum | |
| 1.4 | - |
UDP-alpha-D-glucose | recombinant enzyme expressed in Escherichia coli | Solanum tuberosum | |
| 5 | - |
D-fructose | recombinant enzyme expressed in Saccharomyces cerevisiae | Solanum tuberosum | |
| 5.5 | - |
D-fructose | recombinant enzyme expressed in Escherichia coli | Solanum tuberosum | |
| 60 | - |
sucrose | recombinant enzyme expressed in Saccharomyces cerevisiae | Solanum tuberosum | |
| 80.3 | - |
sucrose | recombinant enzyme expressed in Escherichia coli | Solanum tuberosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose | Solanum tuberosum | - |
UDP + sucrose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | P10691 | sus1; gene sus1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme is phosphorylated at Ser11 | Solanum tuberosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes 450fold from Escherichia coli and 25fold from Saccharomyces cerevisiae by ion exchange and immobilized metal affinity chromatography, and gel filtration | Solanum tuberosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
crude extract, recombinant enzyme in Escherichia coli | Solanum tuberosum |
| 0.3 | - |
crude extract, recombinant enzyme in Saccharomyces cerevisiae | Solanum tuberosum |
| 4.5 | - |
purified recombinant enzyme, expressed in Escherichia coli | Solanum tuberosum |
| 6.9 | - |
purified recombinant enzyme, expressed in Saccharomyces cerevisiae | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificities of recombinant wild-type and mutant enzymes expressed in Escherichia coli or in Saccharomyces cerevisia, overview | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + 1-deoxy-1-fluoro-fructose | the recombinant enzyme expressed in Escherichia coli shows 175% of the activity with D-fructose, 100% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-fructose | - |
Solanum tuberosum | UDP + sucrose | - |
r | |
| UDP-alpha-D-glucose + D-glucose | the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-lyxose | the recombinant enzyme expressed in Escherichia coli shows 150% of the activity with D-fructose, 48% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-mannose | the recombinant enzyme expressed in Escherichia coli shows 75% of the activity with D-fructose, 40% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-ribose | the recombinant enzyme expressed in Sacchromyces cerevisiae shows 7% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-ribulose | the recombinant enzyme expressed in Escherichia coli shows 24% of the activity with D-fructose, no activity with enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-tagatose | the recombinant enzyme expressed in Escherichia coli shows 43% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-talose | the recombinant enzyme expressed in Sacchromyces cerevisiae shows 117% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-xylose | the recombinant enzyme expressed in Escherichia coli shows 300% of the activity with D-fructose, 14% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + D-xylose | the recombinant enzyme expressed in Sacchromyces cerevisiae shows 42% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-arabinose | the recombinant enzyme expressed in Escherichia coli shows 490% of the activity with D-fructose, 36% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-galactose | the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-glucose | the recombinant enzyme expressed in Escherichia coli shows 34% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-mannose | the recombinant enzyme expressed in Escherichia coli shows 59% of the activity with D-fructose, 8% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-rhamnose | the recombinant enzyme expressed in Escherichia coli shows 52% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
| UDP-alpha-D-glucose + L-sorbose | the recombinant enzyme expressed in Escherichia coli shows 96% of the activity with D-fructose, 55% for the enzyme expressed in Sacchromyces cerevisiae | Solanum tuberosum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sucrose synthase 1 | - |
Solanum tuberosum |
| SuSy1 | - |
Solanum tuberosum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
- |
Solanum tuberosum | 2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine | |
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