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Literature summary for 2.4.1.13 extracted from

  • Matic, S.; Akerlund, H.E.; Everitt, E.; Widell, S.
    Sucrose synthase isoforms in cultured tobacco cells (2004), Plant Physiol. Biochem., 42, 299-306.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
actin activity of SuSy2 in breakdown direction is stimulated by 60% Nicotiana tabacum
fructose 2,6-bisphosphate stimulates SuSy2 in presence of actin Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
actin 0.001 mM, 17% inhibition of SuSy1 Nicotiana tabacum
D-fructose 10 mM, about 80% loss of activity, SuSy1 Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
UDP 25°C, isoform SuSy1 Nicotiana tabacum
0.032
-
UDP 25°C, isoform SuSy2 Nicotiana tabacum
0.42
-
UDP-glucose 25°C, isoform SuSy1 Nicotiana tabacum
0.45
-
UDP-glucose 25°C, isoform SuSy2 Nicotiana tabacum
1.05
-
D-fructose 25°C, isoform SuSy1 Nicotiana tabacum
2.65
-
D-fructose 25°C, isoform SuSy2 Nicotiana tabacum
22.3
-
sucrose 25°C, isoform SuSy1 Nicotiana tabacum
30.3
-
sucrose 25°C, isoform SuSy2 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoskeleton associated to Nicotiana tabacum 5856
-
cytosol
-
Nicotiana tabacum 5829
-
membrane bound to Nicotiana tabacum 16020
-

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoform SuSy1 and SuSy2 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP + sucrose
-
Nicotiana tabacum UDP-glucose + D-fructose
-
r

Synonyms

Synonyms Comment Organism
SuSy
-
Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.97
-
sucrose SuSy1, 25°C Nicotiana tabacum
39.5
-
sucrose SuSy2, 25°C Nicotiana tabacum