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Literature summary for 2.4.1.13 extracted from

  • Morell, M.; Copeland, L.
    Sucrose synthase of soybean nodules (1985), Plant Physiol., 78, 149-154.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.13

Activating Compound

Activating Compound Comment Organism Structure
allantoin activation, sucrose synthesis Glycine max

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Glycine max

Inhibitors

Inhibitors Comment Organism Structure
AMP not inhibitory Glycine max
ATP sucrose synthesis Glycine max
fructose
-
 Glycine max
glucose
-
 Glycine max
imidazole
-
 Glycine max
additional information not inhibitory: DTT, GSH, 2-mercaptoethanol, EDTA; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate; not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate Glycine max
Tris sucrose cleavage Glycine max
UDP strong Glycine max
UDPglucose
-
 Glycine max
UTP synthesis Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 UDP
-
 Glycine max
0.012 0.033 UDPglucose
-
 Glycine max
0.13
-
 ADP
-
 Glycine max
0.98 1.66 fructose cosubstrate CDP Glycine max
0.98 1.66 fructose cosubstrate ADPglucose Glycine max
3.7 5 fructose
-
 Glycine max
16.9 40 sucrose
-
 Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Glycine max 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation of sucrose synthesis Glycine max
Ca2+ slight activation of sucrose cleavage at 10 mM Glycine max
Mg2+ activation Glycine max
Mg2+ slight activation of sucrose cleavage Glycine max
Mn2+ activation Glycine max
additional information no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 4 * 90000, SDS-PAGE Glycine max
380000
-
 SDS-PAGE Glycine max
412000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 Merr cv. Williams
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
nodule nodulaid inoculum: Rhizobium japonicum CB 1809 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.1
-
-
 Glycine max

Storage Stability

Storage Stability Organism
4°C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + D-fructose
-
 Glycine max ADP + sucrose
-
 r
CDP-glucose + D-fructose
-
 Glycine max CDP + sucrose
-
 r
GDP-glucose + D-fructose
-
 Glycine max GDP + sucrose
-
 r
UDP + sucrose also substrate: ADP Glycine max UDP-glucose + D-fructose
-
 ?
UDP + sucrose substrate: CDP Glycine max UDP-glucose + D-fructose
-
 ?
UDP + sucrose ADP is less effective Glycine max UDP-glucose + D-fructose
-
 ?
UDPglucose + D-fructose
-
 Glycine max UDP + sucrose
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 90000, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 sucrose cleavage Glycine max
9.5
-
 sucrose synthesis Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5 8.2 about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage Glycine max
7.5 10 about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis Glycine max