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Literature summary for 2.4.1.129 extracted from

  • Morlot, C.; Uehara, T.; Marquis, K.; Bernhardt, T.; Rudner, D.
    A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis (2010), Genes Dev., 24, 411-422.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
E78A the mutant shows 89% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
E88A the mutant is severely impaired in sporulation efficiency (0.01% efficiency compared to the wild type enzyme) Bacillus subtilis
E96A the mutant shows wild type sporulation efficiency Bacillus subtilis
H297A the mutant is severely impaired in sporulation efficiency (0.02% efficiency compared to the wild type enzyme) Bacillus subtilis
K99A the mutant shows wild type sporulation efficiency Bacillus subtilis
Q101A the mutant shows 89% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
Q303A the mutant shows 88% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
R106A the mutant is severely impaired in sporulation efficiency (0.05% efficiency compared to the wild type enzyme) Bacillus subtilis
R269A the mutant shows 86% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
S276A the mutant shows 93% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
T164A the mutant shows 73% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
T188A the mutant shows 33% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
Y171A the mutant shows 71% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
Y201A the mutant shows 87% sporulation efficiency compared to the wild type enzyme Bacillus subtilis
Y323A the mutant is severely impaired in sporulation efficiency (0.01% efficiency compared to the wild type enzyme) Bacillus subtilis
Y324A the mutant is severely impaired in sporulation efficiency (0.02% efficiency compared to the wild type enzyme) Bacillus subtilis
Y80A the mutant shows 70% sporulation efficiency compared to the wild type enzyme Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Bacillus subtilis 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis the enzyme is both an amidase and an endopeptidase. The enzyme is a lytic transglycosylase that degrades the glycan strands of the peptidoglycan into disaccharide units. The enzyme binds the cell wall, but only cleaves the glycan strands after the stem peptides have been removed. The enzyme alone is unable to cleave purified peptidoglycan but needs the presence of SpoIIP ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-agarose column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is both an amidase and an endopeptidase. The enzyme is a lytic transglycosylase that degrades the glycan strands of the peptidoglycan into disaccharide units. The enzyme binds the cell wall, but only cleaves the glycan strands after the stem peptides have been removed. The enzyme alone is unable to cleave purified peptidoglycan but needs the presence of SpoIIP Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
SpoIID
-
Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme also functions as an activity enhancer of SpoIIP which generates its substrate Bacillus subtilis