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Literature summary for 2.4.1.122 extracted from
- Purification, characterization, and subunit structure of rat core 1 beta1,3-galactosyltransferase (2002), J. Biol. Chem., 277, 169-177.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | slight inhibition | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
UDP-galactose | with N-acetyl-D-galactosaminyl-alpha-phenyl | Rattus norvegicus | |
| 0.76 | - |
N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | 86000 | gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + glycoprotein N-acetyl-D-galactosamine | Rattus norvegicus | key enzyme in biosynthesis of core 1 O-glycans | UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on immobilized asialo-bovine submaxillary gland mucin | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | high content | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000042 | - |
liver | Rattus norvegicus |
| 3.43 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + asialo bovine submaxillary mucin | - |
Rattus norvegicus | UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo bovine submaxillary mucin | - |
? | |
| UDP-galactose + glycoprotein N-acetyl-D-galactosamine | linked to serine or threonine of the peptide/protein | Rattus norvegicus | UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine | i.e. core 1 structure | ? | |
| UDP-galactose + glycoprotein N-acetyl-D-galactosamine | key enzyme in biosynthesis of core 1 O-glycans | Rattus norvegicus | UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine | - |
? | |
| UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Rattus norvegicus | UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000-43000, SDS-PAGE and amino acid sequence determination | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| core 1 beta3-Gal-T | - |
Rattus norvegicus |
| UDP-Gal:GalNAc-alpha-Ser/Thr beta3-galactosyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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