Cloned (Comment) | Organism |
---|---|
gene GYS1, in silico cloning, DNA and amino acid sequence determination and analysis, promoter determination and analysis, quantitative real-time RT-PCR tissue expression analysis | Sus scrofa |
gene GYS2, in silico cloning, DNA and amino acid sequence determination and analysis, promoter determination and analysis, quantitative real-time RT-PCR tissue expression analysis | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80600 | - |
x * 80600, about, GYS2, sequence calculation | Sus scrofa |
83700 | - |
x * 83700, about, GYS1, sequence calculation | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + [(1->4)-alpha-D-glucosyl]n | Sus scrofa | - |
UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
UDP-glucose + [(1->4)-alpha-D-glucosyl]n | Sus scrofa Meishan | - |
UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | E0X6R2 | GYS1; gene GYS1 | - |
Sus scrofa | E0X6R4 | GYS2; gene GYS2 | - |
Sus scrofa Meishan | E0X6R2 | GYS1; gene GYS1 | - |
Sus scrofa Meishan | E0X6R4 | GYS2; gene GYS2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | regulatory sites of GYS1 that undergo reversible phosphorylation are identified: two of them, sites 2 and 2a, are located near the N-terminus, while the remaining seven sites, 3a, 3b, 3c, 4, 5, 1a, and 1b, are found within the C-terminus. Insulin promotes dephosphorylation of glycogen synthase in differentiated porcine satellite cells | Sus scrofa |
phosphoprotein | regulatory sites of GYS2 that undergo reversible phosphorylation are identified: two of them, sites 2 and 2a, are located near the N-terminus, while the remaining seven sites, 3a, 3b, 3c, 4, and 5 are found within the C-terminus, GYS2 does not contain sites 1a and 1b | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | subcutaneous, expression of GYS2 | Sus scrofa | - |
adipose tissue | subcutaneous, GYS1 | Sus scrofa | - |
brain | GYS1 | Sus scrofa | - |
heart | high expression level of GYS1 | Sus scrofa | - |
kidney | GYS1 | Sus scrofa | - |
liver | GYS1 | Sus scrofa | - |
liver | high expression level of GYS2 | Sus scrofa | - |
lung | GYS1 | Sus scrofa | - |
additional information | tissue expression pattern of GYS1, GYS1 is upregulated during porcine myogenic cell differentiation | Sus scrofa | - |
additional information | tissue expression pattern of GYS2 | Sus scrofa | - |
ovary | GYS1 | Sus scrofa | - |
satellite cell | GYS1 | Sus scrofa | - |
skeletal muscle | longissimus dorsi muscle, high expression level of GYS1 | Sus scrofa | - |
spleen | GYS1 | Sus scrofa | - |
stomach | GYS1 | Sus scrofa | - |
uterus | GYS1 | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + [(1->4)-alpha-D-glucosyl]n | - |
Sus scrofa | UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
UDP-glucose + [(1->4)-alpha-D-glucosyl]n | - |
Sus scrofa Meishan | UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80600, about, GYS2, sequence calculation | Sus scrofa |
? | x * 83700, about, GYS1, sequence calculation | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
GYS1 | - |
Sus scrofa |
GYS2 | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | GYS1, sequence calculation | - |
5.78 |
Sus scrofa | GYS2, sequence calculation | - |
6.46 |
Organism | Comment | Expression |
---|---|---|
Sus scrofa | insulin does not significantly affect the transcription of GYS1 | additional information |
Sus scrofa | expression level of GYS1 is upregulated from proliferation to differentiation in the porcine satellite cells | up |
General Information | Comment | Organism |
---|---|---|
physiological function | glycogen synthase catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in liver and muscle, phosphorylation sites regulate the activity of the enzyme | Sus scrofa |
physiological function | glycogen synthase catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in liver and muscle, phosphorylation sites regulate the activity of the enzyme. Transcriptional regulation of GYS1 during myogenic differentiation in porcine satellite cells, overview. Effect of insulin is primarily mediated via posttranscriptional control the porcine glycogen synthase. Insulin promotes dephosphorylation of glycogen synthase in differentiated porcine satellite cells | Sus scrofa |