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Literature summary for 2.4.1.109 extracted from

  • Loibl, M.; Strahl, S.
    Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily (2013), Methods Mol. Biol., 1022, 107-117.
    View publication on PubMed
Search for more literature for 2.4.1.109

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of HA-tagged isozyme Pmt1p. In Saccharomyces cerevisiae, C-terminal fusions with HA-, FLAG-, or GFP-epitopes do not affect PMTs mannosyltransferase activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum Pmt1p membrane topology Saccharomyces cerevisiae 5783
-
membrane
-
 Saccharomyces cerevisiae 16020
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P33775
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl D-mannosyl phosphate + YATAVK-Btn photocross-linking reaction based on the peptide YATAVK-Btn that serves as in vitro mannosyl acceptor substrate of Pmt1p/Pmt2p and is O -mannosylated to a similar extent as the known acceptor peptide AcNH-YATAV-CONH2 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosyl-YATAVK-Btn
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
dolichyl phosphate-mannose:protein O-mannosyltransferase
-
 Saccharomyces cerevisiae
PMT
-
 Saccharomyces cerevisiae
Pmt1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
evolution PMT family members are conserved throughout the fungal and animal kingdoms. They are further subdivided into the PMT1, PMT2, and PMT4 subfamilies, which include transferases closely related to Saccharomyces cerevisiae Pmt1p, Pmt2p, and Pmt4p, respectively. In fungi, at least one member of each subfamily is present, whereas in animals only PMT2 and PMT4 subfamily members are conserved. In Saccharomyces, Pmt1p, Pmt2p, and Pmt4p account for the major transferase activities although at least six PMTs (Pmt1p-Pmt6p) are present Saccharomyces cerevisiae
physiological function Dol-P-beta-Man:protein alpha-mannosyltransferases are initiating the essential protein O-mannosylation of secretory and membrane proteins in the endoplasmic reticulum. Pmt1p-loop1 is crucial for cross-linking of the photoreactive peptide Saccharomyces cerevisiae