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Literature summary for 2.4.1.109 extracted from
- Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans (2009), Eukaryot. Cell, 8, 1465-1474.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCR-amplification, introduction of replacement cassettes to disrupt genes | Aspergillus nidulans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Anpmta + AnpmtB double disruptant is viable but very slow growing with morphological characteristics (swollen hyphae with ballon structures + hyperbranched hyphae) cumulative of single disruptants at 30 and 42°C, partial improvement of defects by addition of osmotic stabilizer | Aspergillus nidulans |
| additional information | AnpmtA disruptant, increased sensitivity to hygromycin B (increased cell wall permeability), glycoprotetin profile (plasma membrane proteins) almost indistinguishable from wild-type, underglycosylation of protein AN5660 upon AnpmtC diruption | Aspergillus nidulans |
| additional information | AnPmtB disruptant shows wild-type colony formation at 30°C, slightly repressed growth at 42°C, the osmotic stabilizer 0.6 M KCl reduces these effects, conidiation reduced to about 56%, swollen vesicles, hyperbranching of hyphae (defect in polarity maintenance), underglycosylation of glucoamylase I upon gene disruption, higher sensitivity to inhibitors of cell wall synthesis (beta-glucan and beta-1,3-glucan production) congo red and micafungin, not to calcofluor (inhibiting chitin synthesis), no increased sensitivity to hygromycin B, glycoproetin profile (plasma membrane proteins) almost indistinguishable from wild-type, underglycosylation of glucoamylase I upon AnpmtB diruption | Aspergillus nidulans |
| additional information | AnpmtC disruptant with highest growth repression, swollen, frequently branched hyphae, strongly reduced conidia formation (6% of wild-type), recovery of hyphal structures in the presence of osmotic stabilizers (0.6 M KCl, 0.8 M NaCl, or 1.2 M sorbitol) at 42°C, enables conidiophore and conidia production (abnormal and fewer), underglycosylation of glucoamylase I upon gene diruption, higher sensitivity to inhibitors of cell wall synthesis (beta-glucan and beta-1,3-glucan production) congo red and micafungin, not to calcofluor (inhibiting chitin synthesis), more sensitive to hygromycin B than wild-type, increase of 65 kDa proteins, of mannose-containing glycoproteins (plasma membrane proteins) of about 80 kDa, reduced number of proteins larger than 100 kDa and of 75 kDa, underglycosylation of glucoamylase I and of protein AN5660 upon AnpmtC diruption | Aspergillus nidulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88200 | - |
AnPmtC, calculated from amino acid sequence | Aspergillus nidulans |
| 103300 | - |
AnPmtB, calculated from amino acid sequence | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + glucoamylase I | extracellular Aspergillus awamori protein as reporter for glycosylation measurements, underglycosylation upon AnpmtA, AnpmtB, and AnpmtC diruption | Aspergillus nidulans | dolichyl phosphate + O-D-mannosyl-[glucoamylase] | - |
? |  |
| dolichyl phosphate D-mannose + protein AN5660 | triple-hemagglutin-tagged AN5660.3 protein is an ortholog of Wsc1 protein of Saccharomyces cerevisiae, a sensor for cell wall stress, underglycosylation upon AnpmtA and AnpmtC diruption not due to AnpmtB disruption | Aspergillus nidulans | dolichyl phosphate + O-D-mannosylprotein | AN5660 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | AnpmtB with AnpmtA, 2 * ? | Aspergillus nidulans |
| heterodimer | AnpmtB with AnpmtA, but AnPmtB disruption mutant shows different phenotype than AnpmtA disruptant, independent function | Aspergillus nidulans |
| homodimer | - |
Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AnPmtA | PMT2 subfamily | Aspergillus nidulans |
| AnPmtB | PMT1 subfamily | Aspergillus nidulans |
| AnPmtC | PMT4 subfamily | Aspergillus nidulans |
| O-mannosyltransferase | - |
Aspergillus nidulans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | AnpmtA + AnpmtB double disruptant is viable but slow growing with morphological characteristics cumulative of single disruptants | Aspergillus nidulans |
| malfunction | AnpmtA + AnpmtB double disruptant is viable but slow growing with morphological characteristics cumulative of single disruptants, hyperglycosylation of specific target proteins | Aspergillus nidulans |
| malfunction | AnpmtB disruptant shows wild-type colony formation at 30°C, slightly repressed growth at 42°C, conidiation reduced to about 50%, hyperbranching of hyphae (defect in polarity maintenance) | Aspergillus nidulans |
| malfunction | pmtC disruptant with highest growth repression, swollen, frequently branched hyphae, no conidia formation, recovery of hyphal structures in the presence of osmotic stabilizers, enables conidiophore and conidia production (abnormal and fewer) at 42°C | Aspergillus nidulans |
| physiological function | AnpmtB, hyphal development and differentiation, polarity maintenance | Aspergillus nidulans |
| physiological function | AnpmtC, hyphal development and differentiation, maintenance of cell wall integrity | Aspergillus nidulans |
| physiological function | fungal morphology and conidia formation | Aspergillus nidulans |
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