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Literature summary for 2.4.1.109 extracted from
- Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer (1995), FEBS Lett., 377, 128-130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and overexpression of PMT2 in yeast strain GFUII-4B, showing no alteration of enzyme activity, and co-overexpression with PMT1 in yeast strain TF1.8, leading to 3fold increase in enzyme activity in vitro, thus PMT1 and 2 function as a complex | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 78000, PMT2, SDS-PAGE | Saccharomyces cerevisiae |
| 92000 | - |
x * 92000, PMT1, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene PMT1 | - |
| Saccharomyces cerevisiae | - |
gene PMT2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immunoaffinity chromatography, co-purification of PMT1 and PMT2 as a complex, no immuno-cross reactivity | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + Ac-Tyr-Ala-Thr-Ala-Val-NH2 | recombinant yeast overproducing PMT1 and PMT2 | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Ala-Thr-Ala-Val-NH2 | - |
ir | |
| dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
ir | |
| dolichyl phosphate D-mannose + AcSSSSSNH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-AcSSSSSNH2 | - |
ir | |
| dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
| dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
ir | |
| additional information | PMT1 and PMT2 function as a complex | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, PMT2, SDS-PAGE | Saccharomyces cerevisiae |
| ? | x * 92000, PMT1, SDS-PAGE | Saccharomyces cerevisiae |
| More | PMT1 and PMT2 function as a complex | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMT | - |
Saccharomyces cerevisiae |
| protein O-mannosyltransferase | - |
Saccharomyces cerevisiae |
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