Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.10 extracted from

  • Sunitha, K.; Chung, B.H.; Jang, K.H.; Song, K.B.; Kim, C.H.; Rhee, S.K.
    Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli (2000), Protein Expr. Purif., 18, 388-393.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
constitutive overexpression in Escherichia coli DH5alpha under control of Rahnella aquatilis levansucrase promotor Zymomonas mobilis
expression in Escherichia coli as His-tagged protein Zymomonas mobilis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis Q60114
-
-
Zymomonas mobilis ZM1 Q60114
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant as soluble His-tagged protein, small scale Zymomonas mobilis
recombinant from inclusion bodies in E. coli cytoplasm, direct refolding and solubilization with Triton X-100, large scale in fed-batch culture of recombinant E. coli Zymomonas mobilis

Renatured (Commentary)

Renatured (Comment) Organism
refolding after treatment of inclusion bodies with high conecntrations of urea and guanidine hydrochloride is not successful, formation of large amounts of insoluble aggregates Zymomonas mobilis
refolding and solubilization of recombinant enzyme from inclusion bodies in E. coli by Triton X-100 4% v/v, maximal at pH 6.4-6.8 with 0.3-0.5 M glycine, which enhanced the refolding efficiency Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n
-
Zymomonas mobilis ZM1 glucose + (2,6-beta-D-fructosyl)n+1
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Zymomonas mobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Zymomonas mobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Zymomonas mobilis

pH Range

pH Minimum pH Maximum Comment Organism
5 8.4 recombinant enzyme Zymomonas mobilis