Cloned (Comment) | Organism |
---|---|
constitutive overexpression in Escherichia coli DH5alpha under control of Rahnella aquatilis levansucrase promotor | Zymomonas mobilis |
expression in Escherichia coli as His-tagged protein | Zymomonas mobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | Q60114 | - |
- |
Zymomonas mobilis ZM1 | Q60114 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant as soluble His-tagged protein, small scale | Zymomonas mobilis |
recombinant from inclusion bodies in E. coli cytoplasm, direct refolding and solubilization with Triton X-100, large scale in fed-batch culture of recombinant E. coli | Zymomonas mobilis |
Renatured (Comment) | Organism |
---|---|
refolding after treatment of inclusion bodies with high conecntrations of urea and guanidine hydrochloride is not successful, formation of large amounts of insoluble aggregates | Zymomonas mobilis |
refolding and solubilization of recombinant enzyme from inclusion bodies in E. coli by Triton X-100 4% v/v, maximal at pH 6.4-6.8 with 0.3-0.5 M glycine, which enhanced the refolding efficiency | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis ZM1 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.4 | recombinant enzyme | Zymomonas mobilis |