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Literature summary for 2.4.1.10 extracted from
- Levansucrase from Zymomonas mobilis (1983), Biotechnol. Lett., 5, 345-350.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| aspartic acid | 5 mM, activates partially purified enzyme | Zymomonas mobilis |  |
| fructose 1,6-biphosphate | 5 mM, activates partially purified enzyme | Zymomonas mobilis | |
| glucoseamine-HCl | 5 mM, activates partially purified enzyme | Zymomonas mobilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-phosphogluconate | reaction intermediate | Zymomonas mobilis | |
| D-glucose | complete inhibition at 30 mM | Zymomonas mobilis | |
| ethanol | complete inhibition at 1.6 M | Zymomonas mobilis | |
| Hg2+ | no inhibition by HgCl2, 5 mM | Zymomonas mobilis | |
| mannose | - |
Zymomonas mobilis | |
| additional information | not affected by 2-mercaptoethanol; not affected by cysteine; not affected by several hexoses, overview | Zymomonas mobilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
sucrose | - |
Zymomonas mobilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by 5 mM of Ba2+, Zn2+, Hg2+, Ni2+, Mn2+, Cu2+, Co2+, Ca2+, Na2MoO4 | Zymomonas mobilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Zymomonas mobilis | 6-beta-D-fructosyl)n is levan | ? | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | Zymomonas mobilis | - |
glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Zymomonas mobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Zymomonas mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | 6-beta-D-fructosyl)n is levan | Zymomonas mobilis | ? | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | enzyme plays an important role in the production of ethanol from sugar cane and other sucrose sources | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Zymomonas mobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
hydrolysis of sucrose to glucose and fructose | Zymomonas mobilis |
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Release 2023.1 (January 2023)
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