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Literature summary for 2.4.1.1 extracted from

  • Kyriakis, E.; Stravodimos, G.A.; Kantsadi, A.L.; Chatzileontiadou, D.S.; Skamnaki, V.T.; Leonidas, D.D.
    Natural flavonoids as antidiabetic agents. The binding of gallic and ellagic acids to glycogen phosphorylase b (2015), FEBS Lett., 589, 1787-1794.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP allosteric activator Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
determination of crystal structures of the glycogen phosphorylase b-gallic acid and glycogen phosphorylase b-ellagic acid complexes, overview Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Caffeine
-
Oryctolagus cuniculus
chrysin
-
Oryctolagus cuniculus
ellagic acid competitive inhibitor with respect to the substrate, glucose-1-phoshate, and non-competitive to the allosteric activator, AMP. Ellagic acid functions with glucose in a strongly synergistic mode. Determination of crystal structures of the GPb-gallic acid and GPb-ellagic acid complexes, overview Oryctolagus cuniculus
flavopiridol
-
Oryctolagus cuniculus
gallic acid competitive inhibitor with respect to the substrate, glucose-1-phoshate, and non-competitive to the allosteric activator, AMP Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate Oryctolagus cuniculus
-
[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P00489
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein glycogen phosphorylase a is prepared from glycogen phosphorylase b by phosphorylation through a truncated form of the catalytic subunit of rabbit skeletal muscle phosphorylase kinase Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
native glycogen phosphorylase b from skeletal muscle, glycogen phosphorylase a is prepared from glycogen phosphorylase b by phosphorylation Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate
-
Oryctolagus cuniculus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Synonyms

Synonyms Comment Organism
glycogen phosphorylase b
-
Oryctolagus cuniculus
GPA
-
Oryctolagus cuniculus
GPb
-
Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at, glycogen synthesis Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at, glycogen synthesis Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.73
-
ellagic acid pH 6.8, 30°C Oryctolagus cuniculus
13.4
-
gallic acid pH 6.8, 30°C Oryctolagus cuniculus