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Literature summary for 2.4.1.1 extracted from
- Rice endosperm-specific plastidial alpha-glucan phosphorylase is important for synthesis of short-chain malto-oligosaccharides (2010), Arch. Biochem. Biophys., 495, 82-92.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose 1-phosphate | production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate | Oryza sativa |  |
| phosphate | production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate | Oryza sativa | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged Pho1 in Eschericia coli EA3457 cells | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-glucose | 2fold inhibition at 4 mM | Oryza sativa | |
| Al3+ | - |
Oryza sativa | |
| ATP | slight inhibition | Oryza sativa | |
| Co2+ | - |
Oryza sativa | |
| CTP | slight inhibition | Oryza sativa | |
| Cu2+ | - |
Oryza sativa | |
| Fe2+ | - |
Oryza sativa | |
| GTP | slight inhibition | Oryza sativa | |
| Hg2+ | - |
Oryza sativa | |
| phosphate | Pho1 activity is strongly competitively inhibited by product phosphate in the synthesis reaction when amylopectin is the primer substrate, but this inhibition is less pronounced when short alpha-glucan chains are used as primers | Oryza sativa | |
| phosphoenol pyruvate | slight inhibition | Oryza sativa | |
| Sn2+ | - |
Oryza sativa | |
| Zn2+ | - |
Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | Pho1 is plastidial | Oryza sativa | 9507 | - |
| cytosol | Pho2 | Oryza sativa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates Pho1 activity by 1.5-1.8fold | Oryza sativa | |
| Mg2+ | stimulates Pho1 activity by 1.5-1.8fold | Oryza sativa | |
| Mn2+ | stimulates Pho1 activity by 1.5-1.8fold | Oryza sativa | |
| additional information | Na+, K+, NH4-, and Li+ have essentially no effect on Pho1 activity, and no effect by 4 mM DTT | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Oryza sativa | Pho1 not only degrades maltohexaose but also extends them to synthesize longer malto-oligosaccharides, production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate | ? | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | Oryza sativa | - |
[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q9ATK9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | Pho1 is endosperm-specific | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pho1 not only degrades maltohexaose but also extends them to synthesize longer malto-oligosaccharides, production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate | Oryza sativa | ? | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Oryza sativa | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | usage of four different assay methods | Oryza sativa | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucan phosphorylase | - |
Oryza sativa |
| Pho1 | - |
Oryza sativa |
| type L alpha-glucan phosphorylase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | sharp decline in activity below pH 6.0 and above pH 7.5 | Oryza sativa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
phosphate | Pho1 with amylopectin as primer in the reaction, pH 6.5, 22°C | Oryza sativa | |
| 14.2 | - |
phosphate | Pho1 with short alpha-glucan chain as primer in the reaction, pH 6.5, 22°C | Oryza sativa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | plants contain two major Pho forms: the plastidial Pho1 or PhoL (low glycogen affinity) encoded by the PHO1 gene and a cytosolic Pho2 or PhoH (high glycogen affinity) encoded by the PHO2 gene | Oryza sativa |
| physiological function | the endosperm-specific plastidial alpha-glucan phosphorylase is important for synthesis of short-chain malto-oligosaccharides, it has an essential role during the initiation process of starch biosynthesis during rice seed development | Oryza sativa |
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