Protein Variants | Comment | Organism |
---|---|---|
L31A | mutation decreases the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding | Corynebacterium callunae |
R141A | mutation disrupts the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding | Corynebacterium callunae |
S174A | mutation decreases the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding | Corynebacterium callunae |
T28A | mutation disrupts the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding | Corynebacterium callunae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium callunae | Q8KQ56 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
30°C, pH 7.0, mutant R141A | Corynebacterium callunae |
29 | - |
30°C, pH 7.0, mutant K31A | Corynebacterium callunae |
29 | - |
30°C, pH 7.0, mutant S174A | Corynebacterium callunae |
29 | - |
30°C, pH 7.0, mutant T28A | Corynebacterium callunae |
31 | - |
30°C, pH 7.0, wild-type | Corynebacterium callunae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + phosphate | - |
Corynebacterium callunae | ? + alpha-D-glucose 1-phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life 2.6 min for wild-type, 0.8 min for mutant T28A, 5.3 min for mutant K31A, 2.8 min for mutant S174A, 2.9 min for mutant R141A. In presence of 10 mM phosphate, half-life 280 min for wild-type, 2.2 min for mutant T28A, 170 min for mutant K31A, 33 min for mutant S174A, 7.5 min for mutant R141A. In presence of 100 mM phosphate, half-life 3500 min for wild-type, 330 min for mutant T28A, 3500 min for mutant K31A, 990 min for mutant S174A, 1400 min for mutant R141A | Corynebacterium callunae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
phosphate | 30°C, pH 7.0, mutant R141A | Corynebacterium callunae | |
44 | - |
phosphate | 30°C, pH 7.0, mutant K31A | Corynebacterium callunae | |
44 | - |
phosphate | 30°C, pH 7.0, mutant S174A | Corynebacterium callunae | |
44 | - |
phosphate | 30°C, pH 7.0, mutant T28A | Corynebacterium callunae | |
47 | - |
phosphate | 30°C, pH 7.0, wild-type | Corynebacterium callunae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | about 0.7 mol per mol of protein | Corynebacterium callunae |