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Literature summary for 2.4.1.1 extracted from

  • Mizanur, R.M.; Griffin, A.K.; Pohl, N.L.
    Recombinant production and biochemical characterization of a hyperthermostable alpha-glucan/maltodextrin phosphorylase from Pyrococcus furiosus (2008), Archaea, 2, 169-176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31
-
 phosphate 80°C, pH 6.5 Pyrococcus furiosus
31
-
 phosphate pH 6.8, 80°C Pyrococcus furiosus
70
-
 maltotriose 80°C, pH 6.5, direction of glucose 1-phosphate synthesis Pyrococcus furiosus
70
-
 maltotriose pH 6.8, 80°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 mass spectrometry Pyrococcus furiosus
98000
-
 x * 98000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus Q8U0Q0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential bi-bi mechanism Pyrococcus furiosus
a
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential bi-bi catalytic mechanism Pyrococcus furiosus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
 80°C, pH 6.5, substrate glycogen Pyrococcus furiosus
52
-
 80°C, pH 6.5, substrate maltoheptaose Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + alpha-D-glucose 1-phosphate 75% of the activity with maltose, 64% of the activity with maltoheptaose Pyrococcus furiosus glycogen + phosphate
-
 r
glycogen + phosphate
-
 Pyrococcus furiosus ? + alpha-D-glucose 1-phosphate
-
 r
maltoheptaose + alpha-D-glucose 1-phosphate 80% of the activity with maltose Pyrococcus furiosus maltooctaose + phosphate
-
 r
maltoheptaose + phosphate
-
 Pyrococcus furiosus maltohexaose + alpha-D-glucose 1-phosphate
-
 r
maltoheptaose + phosphate 35% of the activity with maltoheptaose Pyrococcus furiosus maltohexaose + alpha-D-glucose 1-phosphate
-
 r
maltohexaose + alpha-D-glucose 1-phosphate 82% of the activity with maltose Pyrococcus furiosus maltoheptaose + phosphate
-
 r
maltohexaose + phosphate
-
 Pyrococcus furiosus maltopentaose + alpha-D-glucose 1-phosphate
-
 r
maltohexaose + phosphate 85% of the activity with maltoheptaose Pyrococcus furiosus maltopentaose + alpha-D-glucose 1-phosphate
-
 r
maltopentaose + alpha-D-glucose 1-phosphate 86% of the activity with maltose Pyrococcus furiosus maltohexaose + phosphate
-
 r
maltopentaose + phosphate
-
 Pyrococcus furiosus maltotetraose + alpha-D-glucose 1-phosphate
-
 r
maltopentaose + phosphate 78% of the activity with maltoheptaose Pyrococcus furiosus maltotetraose + alpha-D-glucose 1-phosphate
-
 r
maltose + alpha-D-glucose 1-phosphate
-
 Pyrococcus furiosus maltotriose + phosphate
-
 r
maltotetraose + alpha-D-glucose 1-phosphate 90% of the activity with maltose Pyrococcus furiosus maltopentaose + phosphate
-
 r
maltotetraose + phosphate
-
 Pyrococcus furiosus maltotriose + alpha-D-glucose 1-phosphate
-
 r
maltotetraose + phosphate 61% of the activity with maltoheptaose Pyrococcus furiosus maltotriose + alpha-D-glucose 1-phosphate
-
 r
maltotriose + alpha-D-glucose 1-phosphate 95% of the activity with maltose Pyrococcus furiosus maltotetraose + phosphate
-
 r
maltotriose + phosphate
-
 Pyrococcus furiosus maltose + alpha-D-glucose 1-phosphate
-
 r
maltotriose + phosphate 35% of the activity with maltoheptaose Pyrococcus furiosus maltose + alpha-D-glucose 1-phosphate
-
 r
starch + phosphate
-
 Pyrococcus furiosus ? + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate broad substrate specificity in both the synthesis and degradation of maltooligosaccharides In the synthetic direction of the enzymatic reaction, the lowest oligosaccharide required for the chain elongation is maltose. In the degradative direction the archaeal enzyme can produce D-glucose-1-phosphate from maltotriose or longer maltooligosaccharides including both glycogen and starch Pyrococcus furiosus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
alpha-glucan/maltodextrin phosphorylase
-
 Pyrococcus furiosus
PF1535 locus name Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 Tris-HCl buffer, 24 h, no loss of activity Pyrococcus furiosus
80
-
 retains its complete activity after 24 h at 80°C in Tris-HCl buffer Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Pyrococcus furiosus