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Literature summary for 2.4.1.1 extracted from
- Immobilization of starch phosphorylase from seeds of Indian millet (Pennisetum typhoides) variety KB 560 (2007), Afr. J. Biotechnol., 6, 2715-2720.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | immobilized enzyme is used for the production of glucose-1-phosphate, a cytostatic compound used in cardiotherapy | Cenchrus americanus |
General Stability
| General Stability | Organism |
|---|---|
| increase in thermostability and reusability of the enzyme immobilzed on brick dust | Cenchrus americanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cenchrus americanus | - |
variety KB 560 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cenchrus americanus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Cenchrus americanus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glucose 1-phosphate | - |
Cenchrus americanus | starch + phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| starch phosphorylase | - |
Cenchrus americanus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
soluble enzyme | Cenchrus americanus |
| 45 | - |
immobilized enzyme | Cenchrus americanus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | 40 | 50% of maximal activity at 34°C and 40°C, soluble enzyme | Cenchrus americanus |
| 38 | 50 | 50% of maximal activity at 34°C and 40°C, immobilized enzyme | Cenchrus americanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
direction of polysaccharide synthesis. Upon immobilization on brick dust, there is a slight alkaline shift in the optimum pH | Cenchrus americanus |
| 7.6 | - |
direction of glucose-1-phosphate formation. Upon immobilization on brick dust, there is a slight alkaline shift in the optimum pH | Cenchrus americanus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7.9 | 50% of maximal activity at pH 5.4 and 7.9, direction of polysaccharide synthesis | Cenchrus americanus |
| 6.7 | 7.9 | 50% of maximal activity at pH 6.7 and 7.9, direction of glucose-1-phosphate formation | Cenchrus americanus |
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