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Literature summary for 2.4.1.1 extracted from
- Facile synthesis of glucose-1-phosphate from starch by Thermus caldophilus GK24 alpha-glucan phosphorylase (2005), Process Biochem., 40, 3707-3713.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of D-glucose-1-phosphate with yield of 47% w/w from a reaction containing 5% w/v soluble starch in 0.7 M potassium phosphate. Purification of D-glucose-1-phosphate by ethanol preciptitation | Thermus caldophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermus caldophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
6 * 91677, calculated, 6 * 90000, SDS-PAGE | Thermus caldophilus |
| 91677 | - |
6 * 91677, calculated, 6 * 90000, SDS-PAGE | Thermus caldophilus |
| 550000 | - |
PAGE and gel filtration | Thermus caldophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus caldophilus | - |
overexpression of enzyme in Escherichia coli | - |
| Thermus caldophilus GK24 | - |
overexpression of enzyme in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli | Thermus caldophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.5 | - |
- |
Thermus caldophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + phosphate | - |
Thermus caldophilus | amylopectin + D-glucose-1-phosphate | - |
? |  |
| amylopectin + phosphate | - |
Thermus caldophilus GK24 | amylopectin + D-glucose-1-phosphate | - |
? | |
| amylose + phosphate | - |
Thermus caldophilus | amylose + D-glucose-1-phosphate | - |
? | |
| amylose + phosphate | - |
Thermus caldophilus GK24 | amylose + D-glucose-1-phosphate | - |
? | |
| starch + phosphate | - |
Thermus caldophilus | starch + D-glucose-1-phosphate | - |
? | |
| starch + phosphate | - |
Thermus caldophilus GK24 | starch + D-glucose-1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 91677, calculated, 6 * 90000, SDS-PAGE | Thermus caldophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlgP | - |
Thermus caldophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermus caldophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
synthesis of glucose-1-phosphate | Thermus caldophilus |
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Release 2023.1 (January 2023)
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