Literature summary for 2.4.1.1 extracted from

Dauvillee, D.; Chochois, V.; Steup, M.; Haebel, S.; Eckermann, N.; Ritte, G.; Ral, J.P.; Colleoni, C.; Hicks, G.; Wattebled, F.; Deschamps, P.; dHulst, C.; Lienard, L.; Cournac, L.; Putaux, J.L.; Dupeyre, D.; Ball, S.G.
Plastidial phosphorylase is required for normal starch synthesis in Chlamydomonas reinhardtii (2006), Plant J., 48, 274-285.

Search for more literature for 2.4.1.1

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme mutants display a significant decrease in amounts of starch during storage that correlates with the accumulation of abnormally shaped granules containing a modified amylopekti structure and a high amylose content	Chlamydomonas reinhardtii

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP-glucose	maximal inhibition achieved is 70%; maximal inhibition achieved is 95%	Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.41	-	alpha-D-glucose 1-phosphate	pH 7.0, 30°C	Chlamydomonas reinhardtii
0.68	-	alpha-D-glucose 1-phosphate	pH 7.0, 30°C	Chlamydomonas reinhardtii
1.6	-	phosphate	pH 7.0, 30°C	Chlamydomonas reinhardtii
1.9	-	phosphate	pH 7.0, 30°C	Chlamydomonas reinhardtii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Chlamydomonas reinhardtii	9536	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
91000	-	2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated	Chlamydomonas reinhardtii
94000	-	2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated	Chlamydomonas reinhardtii
106000	-	2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme	Chlamydomonas reinhardtii
108000	-	2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme	Chlamydomonas reinhardtii
182000	-	gel filtration	Chlamydomonas reinhardtii
214000	-	gel filtration	Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Chlamydomonas reinhardtii	enzyme is involved in storage starch synthesis	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	Q2VA40	isoform PhoA	-
Chlamydomonas reinhardtii	Q2VA41	isoform PhoB, encoded by STA4	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	-	Chlamydomonas reinhardtii
proteolytic modification	cleavage of a 55 amino acid transit peptide	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate	-	Chlamydomonas reinhardtii	(maltooligosaccharide)n + phosphate	-	r
(maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate	preferred substrate	Chlamydomonas reinhardtii	(maltooligosaccharide)n + phosphate	-	r
glycogen + alpha-D-glucose 1-phosphate	-	Chlamydomonas reinhardtii	glycogen + phosphate	-	r
additional information	enzyme is involved in storage starch synthesis	Chlamydomonas reinhardtii	?	-	?
starch + alpha-D-glucose 1-phosphate	-	Chlamydomonas reinhardtii	starch + phosphate	-	r
starch + alpha-D-glucose 1-phosphate	preferred substrate	Chlamydomonas reinhardtii	starch + phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme	Chlamydomonas reinhardtii
dimer	2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated	Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	40	broad	Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	isoform PhoA	Chlamydomonas reinhardtii
7.5	-	-	Chlamydomonas reinhardtii

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.2	-	ADP-glucose	presence of 1 mM orthophosphate, pH 7.0, 30°C	Chlamydomonas reinhardtii
1	-	ADP-glucose	presence of 1 mM orthophosphate, pH 7.0, 30°C	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)