Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.1 extracted from

  • Schupp, N.; Ziegler, P.
    The relation of starch phosphorylases to starch metabolism in wheat (2004), Plant Cell Physiol., 45, 1471-1484.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
amylopectin pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
0.03
-
amylopectin pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
0.04
-
glycogen pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
0.07
-
glycogen pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
0.1
-
soluble starch pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
0.15
-
soluble starch pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
0.44
-
maltotetraose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
0.71
-
amylose pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
0.81
-
maltoheptaose pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
3.01
-
amylose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
4.49
-
maltoheptaose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastidic isozyme Triticum aestivum 9507
-
cytosol cytosolic isozyme Triticum aestivum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91500
-
x * 91500, cytosolic isozyme, SDS-PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1,4-alpha-D-glucosyl)n + phosphate Triticum aestivum the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Triticum aestivum Q9LKJ3 cytosolic isozyme; var. Star, 3 tissue-dependent enzyme isoforms P1, P2, and P3
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic isozyme from leaves 7140fold to homogeneity by affinity chromatography Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
embryo contains only cytosolic isozyme Triticum aestivum
-
endosperm
-
Triticum aestivum
-
leaf young and mature, the latter contain only the plastidic isozyme Triticum aestivum
-
seed germinated seeds contain only the cytosolic isozyme restricted to the embryo Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity of cytosolic isozyme Triticum aestivum
57.2
-
purified cytosolic isozyme Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-glucosyl)n + phosphate the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch Triticum aestivum (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
(1,4-alpha-D-glucosyl)n + phosphate the cytosolic isozyme utilizes amylopectin, amylose, soluble starch, and glycogen as polysaccharide substrates, while the plastidic isozyme prefers starch Triticum aestivum (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
amylopectin + phosphate
-
Triticum aestivum amylopectin + D-glucose-1-phosphate
-
r
glycogen + phosphate
-
Triticum aestivum alpha-D-glucan + alpha-D-glucose 1-phosphate
-
r
maltoheptaose + alpha-D-glucose 1-phosphate the cytosolic isozyme Triticum aestivum maltooctaose + phosphate
-
r
maltoheptaose + phosphate the cytosolic isozyme Triticum aestivum maltohexaose + alpha-D-glucose 1-phosphate
-
r
maltotetraose + alpha-D-glucose 1-phosphate the cytosolic isozyme Triticum aestivum maltopentaose + phosphate
-
r
additional information substrate specificity of cytosolic isozyme Triticum aestivum ?
-
?
soluble starch + phosphate
-
Triticum aestivum ? + alpha-D-glucose 1-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 91500, cytosolic isozyme, SDS-PAGE Triticum aestivum

Synonyms

Synonyms Comment Organism
alpha-1,4-glycan phosphorylase
-
Triticum aestivum
glycogen phosphorylase
-
Triticum aestivum
starch phosphorylase
-
Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
degradation reaction, assay at Triticum aestivum
37
-
synthesis reaction, assay at Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Triticum aestivum