Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
amylopectin | pH 7.5, 30°C, degradation reaction, cytosolic isozyme | Triticum aestivum | |
0.03 | - |
amylopectin | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum | |
0.04 | - |
glycogen | pH 7.5, 30°C, degradation reaction, cytosolic isozyme | Triticum aestivum | |
0.07 | - |
glycogen | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum | |
0.1 | - |
soluble starch | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum | |
0.15 | - |
soluble starch | pH 7.5, 30°C, degradation reaction, cytosolic isozyme | Triticum aestivum | |
0.44 | - |
maltotetraose | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum | |
0.71 | - |
amylose | pH 7.5, 30°C, degradation reaction, cytosolic isozyme | Triticum aestivum | |
0.81 | - |
maltoheptaose | pH 7.5, 30°C, degradation reaction, cytosolic isozyme | Triticum aestivum | |
3.01 | - |
amylose | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum | |
4.49 | - |
maltoheptaose | pH 7.5, 37°C, synthesis reaction, cytosolic isozyme | Triticum aestivum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastidic isozyme | Triticum aestivum | 9507 | - |
cytosol | cytosolic isozyme | Triticum aestivum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91500 | - |
x * 91500, cytosolic isozyme, SDS-PAGE | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | Triticum aestivum | the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | Q9LKJ3 | cytosolic isozyme; var. Star, 3 tissue-dependent enzyme isoforms P1, P2, and P3 | - |
Purification (Comment) | Organism |
---|---|
cytosolic isozyme from leaves 7140fold to homogeneity by affinity chromatography | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | contains only cytosolic isozyme | Triticum aestivum | - |
endosperm | - |
Triticum aestivum | - |
leaf | young and mature, the latter contain only the plastidic isozyme | Triticum aestivum | - |
seed | germinated seeds contain only the cytosolic isozyme restricted to the embryo | Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of cytosolic isozyme | Triticum aestivum |
57.2 | - |
purified cytosolic isozyme | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch | Triticum aestivum | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
(1,4-alpha-D-glucosyl)n + phosphate | the cytosolic isozyme utilizes amylopectin, amylose, soluble starch, and glycogen as polysaccharide substrates, while the plastidic isozyme prefers starch | Triticum aestivum | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
amylopectin + phosphate | - |
Triticum aestivum | amylopectin + D-glucose-1-phosphate | - |
r | |
glycogen + phosphate | - |
Triticum aestivum | alpha-D-glucan + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptaose + alpha-D-glucose 1-phosphate | the cytosolic isozyme | Triticum aestivum | maltooctaose + phosphate | - |
r | |
maltoheptaose + phosphate | the cytosolic isozyme | Triticum aestivum | maltohexaose + alpha-D-glucose 1-phosphate | - |
r | |
maltotetraose + alpha-D-glucose 1-phosphate | the cytosolic isozyme | Triticum aestivum | maltopentaose + phosphate | - |
r | |
additional information | substrate specificity of cytosolic isozyme | Triticum aestivum | ? | - |
? | |
soluble starch + phosphate | - |
Triticum aestivum | ? + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 91500, cytosolic isozyme, SDS-PAGE | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-glycan phosphorylase | - |
Triticum aestivum |
glycogen phosphorylase | - |
Triticum aestivum |
starch phosphorylase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
degradation reaction, assay at | Triticum aestivum |
37 | - |
synthesis reaction, assay at | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Triticum aestivum |