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Literature summary for 2.4.1.1 extracted from
- Purification and characterization of an alpha-glucan phosphorylase from the thermophilic bacterium Thermus thermophilus (1996), Eur. J. Biochem., 239, 150-155.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-glucose-1-methylenephosphonate | competitive vs. glucose 1-phosphate | Escherichia coli |  |
| alpha-D-glucose-1-methylenephosphonate | competitive vs. glucose 1-phosphate | Thermus thermophilus | |
| arsenate | - |
Escherichia coli | |
| arsenate | - |
Thermus thermophilus | |
| gluconolactone | - |
Escherichia coli | |
| gluconolactone | - |
Thermus thermophilus | |
| [(alpha-D-glucopyranosyloxy)methyl]phosphonic acid | competitive vs. glucose 1-phosphate | Escherichia coli | |
| [(alpha-D-glucopyranosyloxy)methyl]phosphonic acid | competitive vs. glucose 1-phosphate | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
maltoheptaose | phosphorolysis | Escherichia coli | |
| 0.5 | - |
phosphate | phosphorolysis | Escherichia coli | |
| 1 | - |
glucose 1-phosphate | synthesis | Escherichia coli | |
| 1.2 | - |
glucose 1-phosphate | synthesis | Thermus thermophilus | |
| 1.7 | - |
maltoheptaose | phosphorolysis, at 30°C | Thermus thermophilus | |
| 1.9 | - |
phosphate | phosphorolysis, at 30°C | Thermus thermophilus | |
| 2.2 | - |
phosphate | phosphorolysis, at 70°C | Thermus thermophilus | |
| 2.8 | - |
maltotetraose | phosphorolysis, at 30°C | Thermus thermophilus | |
| 3.6 | - |
maltotetraose | synthesis | Escherichia coli | |
| 3.6 | - |
maltoheptaose | phosphorolysis, at 70°C | Thermus thermophilus | |
| 3.9 | - |
maltotetraose | phosphorolysis | Escherichia coli | |
| 4.8 | - |
maltotetraose | phosphorolysis, at 70°C | Thermus thermophilus | |
| 12.7 | - |
maltotetraose | synthesis | Thermus thermophilus | |
| 13.4 | - |
maltotriose | synthesis | Thermus thermophilus | |
| 24.5 | - |
maltotriose | synthesis | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
2 * 93000, most likely subunit composition, SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Q-Sepharose, Mono Q, gel filtration | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dextrin)n-1 + glucose 1-phosphate | - |
Thermus thermophilus | (dextrin)n + phosphate | - |
r | |
| (dextrin)n-1 + glucose 1-phosphate | - |
Escherichia coli | (dextrin)n + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | - |
Thermus thermophilus | glycogen + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | - |
Escherichia coli | glycogen + phosphate | - |
r | |
| maltoheptaose + phosphate | - |
Escherichia coli | maltohexaose + alpha-D-glucose 1-phosphate | - |
r | |
| maltohexaose + glucose 1-phosphate | - |
Thermus thermophilus | maltoheptaose + phosphate | - |
r | |
| maltotetraose + glucose 1-phosphate | - |
Thermus thermophilus | maltopentaose + phosphate | - |
r | |
| maltotetraose + phosphate | - |
Escherichia coli | maltotriose + alpha-D-glucose 1-phosphate | - |
r | |
| maltotriose + glucose 1-phosphate | - |
Thermus thermophilus | maltotetraose + phosphate | - |
r | |
| maltotriose + phosphate | - |
Escherichia coli | maltobiose + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Thermus thermophilus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Escherichia coli | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 93000, most likely subunit composition, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
50% activity at 90°C | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life: 10 h | Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
| pyridoxal 5'-phosphate | 1.2 mol/subunit | Thermus thermophilus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
alpha-D-glucose-1-methylenephosphonate | - |
Thermus thermophilus | |
| 0.4 | - |
alpha-D-glucose-1-methylenephosphonate | - |
Escherichia coli | |
| 0.5 | - |
arsenate | - |
Escherichia coli | |
| 0.9 | - |
gluconolactone | - |
Escherichia coli | |
| 3.3 | - |
gluconolactone | - |
Thermus thermophilus | |
| 4.1 | - |
arsenate | - |
Thermus thermophilus | |
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