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Literature summary for 2.4.1.1 extracted from

  • Schliselfeld, L.H.
    Comparative studies of phosphorylase isozymes from the rabbit (1973), Ann. N. Y. Acad. Sci., 210, 181-191.
    View publication on PubMed
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
5'-AMP activation Sus scrofa
5'-AMP activation Oryctolagus cuniculus
5'-AMP phosphorylase Ia, half-maximal activity at 0.25 mM, phosphorylase IIIa, half-maximal activity at 6 mM Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Antibodies to skeletal muscle phosphorylase heart enzyme Oryctolagus cuniculus
ATP complete inhibition of heart phosphorylase IIIb Oryctolagus cuniculus
ATP
-
 Sus scrofa
glucose 6-phosphate complete inhibition of heart phosphorylase IIIb Oryctolagus cuniculus
glucose 6-phosphate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 glucose 1-phosphate heart phosphorylase I and III Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Oryctolagus cuniculus
-
liver
-
 Sus scrofa
-
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 liver phosophorylase b, in the presence of 1 mM AMP Oryctolagus cuniculus
13
-
 liver phosphorylase b, in the presence of 700 mM Na2SO4 Oryctolagus cuniculus
50
-
 liver phosphorylase a Oryctolagus cuniculus
54
-
 heart phosphorylase IIIa Oryctolagus cuniculus
64
-
 heart phosphorylase Ia Oryctolagus cuniculus
70
-
 heart phosphorylase Ib, in the presence of 1 mM AMP Oryctolagus cuniculus
70
-
 liver phosophorylase a, in the presence of 1 mM AMP Oryctolagus cuniculus
80
-
 heart phosphorylase IIIa, in the presence of 1 mM AMP Oryctolagus cuniculus
82
-
 heart phosphorylase Ia, in the presence of 1 mM AMP Oryctolagus cuniculus
88
-
 heart phosphorylase IIIb, in the presence of 1 mM AMP Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
0°C, heart isozyme Ia, stable in the presence of 2-mercaptoethanol Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + glucose 1-phosphate
-
 Sus scrofa glycogen + phosphate
-
 r
glycogen + glucose 1-phosphate
-
 Oryctolagus cuniculus glycogen + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Oryctolagus cuniculus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 heart isozyme Ib or IIIb: t1/2: 2.4-2.9 min, heart isozyme Ia: stable in the presence of 2-mercaptoethanol Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.7 heart isozyme IIIb Oryctolagus cuniculus
6.5 6.9 heart isozyme Ib Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Sus scrofa
pyridoxal 5'-phosphate requirement Oryctolagus cuniculus
pyridoxal 5'-phosphate 2 mol per mol enzyme Oryctolagus cuniculus