Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | activation | Rattus norvegicus | |
5'-AMP | half-maximal activation of phosphorylase Ib at 0.022 mM, 0.007 mM for phosphorylase IIb | Rattus norvegicus | |
phosphate | activation | Rattus norvegicus | |
phosphate | converts phosphorylase b into phosphorylase a | Rattus norvegicus | |
Phosphorylase kinase | activation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Caffeine | 5 mM, 95% inhibition of heart phosphorylase Ib in the presence of 1 mM AMP, 30% inhibition of heart phosphorylase IIb | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | Rattus norvegicus | glycogen metabolism | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | incorporation of approx. 1 mol phosphate/mol of subunit | Rattus norvegicus |
side-chain modification | activation by phosphorylation | Rattus norvegicus |
side-chain modification | phosphorylation converts inactive phosphorylase b into active phosphorylase a | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
heart isozymes I and II, ammonium sulfate, DEAE-Sephacel, AMP-Sepharose, muscle enzyme, ammonium sulfate, DEAE-Sephacel | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
muscle | skeletal muscle | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46 | - |
heart isoenzymes I and II | Rattus norvegicus |
51.6 | - |
skeletal muscle | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, at least 4 months | Rattus norvegicus |
glycerol stabilizes purified enzyme during storage | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | - |
Rattus norvegicus | glycogen + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | glycogen metabolism | Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.2, skeletal muscle, heart isozyme Ib | Rattus norvegicus |
additional information | - |
pI: 5.2, heart isozyme IIb | Rattus norvegicus |