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Literature summary for 2.4.1.1 extracted from
- Purification and some properties of two phosphorylases from Typha latifolia pollen (1988), Agric. Biol. Chem., 52, 407-412.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data | Typha latifolia | |
| 0.12 | - |
maltoheptaose | synthesis, isoenzyme P-2 | Typha latifolia |  |
| 0.18 | - |
maltoheptaose | synthesis, isoenzyme P-2 | Typha latifolia | |
| 0.26 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
| 0.49 | - |
maltopentaose | synthesis, isoenzyme P-2 | Typha latifolia | |
| 0.59 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
| 0.88 | - |
maltotriose | synthesis, isoenzyme P-2 | Typha latifolia | |
| 1.1 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
| 1.7 | - |
maltopentaose | synthesis, isoenzyme P-1 | Typha latifolia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
x * 112000, SDS-PAGE | Typha latifolia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Typha latifolia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes P-1 and P-2, ammonium sulfate, DE-52, amylose-Sepharose, BioGel | Typha latifolia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen grain | mature | Typha latifolia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11 | - |
isozyme P-2 | Typha latifolia |
| 42.3 | - |
isozyme P-1 | Typha latifolia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + glucose 1-phosphate | - |
Typha latifolia | amylose + phosphate | - |
r | |
| maltoheptaose + glucose 1-phosphate | isozyme I | Typha latifolia | maltooctaose + phosphate | - |
r | |
| maltopentaose + glucose 1-phosphate | isozyme I | Typha latifolia | maltohexaose + phosphate | - |
r | |
| maltotriose + alpha-D-glucose 1-phosphate | - |
Typha latifolia | maltotetraose + phosphate | - |
r | |
| starch + glucose 1-phosphate | soluble starch | Typha latifolia | starch + phosphate | - |
r | |
| starch + glucose 1-phosphate | glucosylation of native starch granules occurs at 82%, isozyme P-1, or 11.5%, isozyme P-2, the rate of soluble starch, phosphorolysis at the same rate as soluble starch | Typha latifolia | starch + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Typha latifolia | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 112000, SDS-PAGE | Typha latifolia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 5.8 | isoenzymes P-1 and P-2, phosphorolysis | Typha latifolia |
| 7.3 | 8 | synthesis | Typha latifolia |
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