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Literature summary for 2.4.1.1 extracted from
- Multiple forms of alpha-glucan phosphorylase in banana fruits: properties and kinetics (1976), Phytochemistry, 15, 1447-1451.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | activation | Musa x paradisiaca |  |
| ATP | activation | Musa x paradisiaca | |
| ATP | no activation of phosphorylase A or C | Musa x paradisiaca | |
| EDTA | 5 mM, 46% activation of phosphorylase C, not A or B | Musa x paradisiaca | |
| additional information | not activated by serine, leucine, isoleucine, methionine, ornithine, L-arginine | Musa x paradisiaca |
General Stability
| General Stability | Organism |
|---|---|
| dialysis for 24 h against water, stable to, phosphorylase A or C | Musa x paradisiaca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aromatic amino acid | inhibition of phosphorylase B, not A or C | Musa x paradisiaca | |
| ATP | 1 mM, 20% inhibition of phosphorylase B, 5 mM, 90% inhibition; phosphorylase A or C are not inhibited | Musa x paradisiaca | |
| DL-phenylalanine | 1 mM, 33% inhibition of phosphorylase B | Musa x paradisiaca | |
| DL-tryptophan | 1 mM, 41% inhibition of phosphorylase B | Musa x paradisiaca | |
| Hg2+ | 0.05 mM, 21% inhibition of phosphorylase A, 73% inhibition of phosphorylase | Musa x paradisiaca | |
| L-tyrosine | 1 mM, 48% inhibition of phosphorylase B | Musa x paradisiaca | |
| monohydroxyphenols | 10 mM, 50, 40 and 65% inhibition of phosphorylases A, B and C | Musa x paradisiaca | |
| additional information | not inhibited by 2-mercaptoethanol; not inhibited by non-aromatic amino acids; not inhibited by p-coumaric acid, caffeic acid or cinnamic acid | Musa x paradisiaca | |
| resorcinol | 1 mM, 50% inhibition of phosphorylase A and B, 23% inhibition of phosphorylase C | Musa x paradisiaca | |
| Tannic acid | 0.005 mg, complete inhibition of phosphorylase B, 63% and 50% inhibition of phosphorylase A and C respectively | Musa x paradisiaca | |
| UDPglucose | 1 mM, 43, 27 and 18% inhibition of phosphorylases A, B and C respectively | Musa x paradisiaca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters of 3 starch phosphorylases | Musa x paradisiaca | |
| additional information | - |
additional information | 1.21 mg/ml, starch, phosphorylase C | Musa x paradisiaca | |
| additional information | - |
additional information | 1.1 mg/ml, glycogen, phosphorylase A | Musa x paradisiaca | |
| additional information | - |
additional information | 0.24 mg/ml starch, phosphorylase A | Musa x paradisiaca | |
| 1.4 | - |
glucose 1-phosphate | starch synthesis, phosphorylase A | Musa x paradisiaca | |
| 2.2 | - |
glucose 1-phosphate | maltose synthesis, phosphorylase A | Musa x paradisiaca | |
| 2.7 | - |
glucose 1-phosphate | starch synthesis, phosphorylase C | Musa x paradisiaca | |
| 3.3 | - |
glucose 1-phosphate | starch synthesis, phosphorylase B | Musa x paradisiaca | |
| 4 | - |
glucose 1-phosphate | maltose synthesis, phosphorylase C | Musa x paradisiaca | |
| 5 | - |
glucose 1-phosphate | glycogen synthesis, phosphorylase A | Musa x paradisiaca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by Cu2+ | Musa x paradisiaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musa x paradisiaca | - |
banana, cooking variety | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphorylases A, B and C | Musa x paradisiaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Musa x paradisiaca | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, phosphorylase A or C, at least 1 month | Musa x paradisiaca |
| -20°C, phosphorylase B, at least 1 month | Musa x paradisiaca |
| 0-4°C, at least 1 month | Musa x paradisiaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dextrin)n-1 + glucose 1-phosphate | not phosphorylase B | Musa x paradisiaca | (dextrin)n + phosphate | - |
r | |
| (dextrin)n-1 + glucose 1-phosphate | achrodextrin | Musa x paradisiaca | (dextrin)n + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | not phosphorylase B | Musa x paradisiaca | glycogen + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | glycogen from oyster | Musa x paradisiaca | glycogen + phosphate | - |
r | |
| maltose + alpha-D-glucose 1-phosphate | not phosphorylase B | Musa x paradisiaca | maltotriose + phosphate | - |
? | |
| starch + glucose 1-phosphate | soluble starch | Musa x paradisiaca | starch + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Musa x paradisiaca | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
phosphorylase B | Musa x paradisiaca |
| 45 | - |
phosphorylase A | Musa x paradisiaca |
| 50 | - |
phosphorylase C | Musa x paradisiaca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
phosphorylase B, inactivation above | Musa x paradisiaca |
| 45 | - |
phosphorylase A or C, 30 min stable | Musa x paradisiaca |
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