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Literature summary for 2.4.1.1 extracted from
- Characteristics of alpha-glucan phosphorylase from chlorella vulgaris (1983), Phytochemistry, 22, 835-840.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing inactivates | Chlorella vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 1.6 mM, 50% inhibition of starch phosphorolysis, 0.23 mM, 50% inhibition of starch synthesis, allosteric inhibition, kinetics | Chlorella vulgaris |  |
| ADPglucose | - |
Chlorella vulgaris | |
| ATP | 2.9 mM, 50% inhibition of starch phosphorolysis, 1.4 mM, 50% inhibition of starch synthesis | Chlorella vulgaris | |
| GDP | - |
Chlorella vulgaris | |
| additional information | not inhibited by GMP; not inhibited by TMP; not inhibited by UMP, CMP, CTP | Chlorella vulgaris | |
| UDPglucose | kinetics | Chlorella vulgaris | |
| UTP | - |
Chlorella vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Chlorella vulgaris | |
| 0.12 | - |
glucose 1-phosphate | glucan synthesis | Chlorella vulgaris | |
| 2.4 | - |
phosphate | glucan phosphorolysis | Chlorella vulgaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Chlorella vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, at least 3 months | Chlorella vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + glucose 1-phosphate | potato amylopectin | Chlorella vulgaris | amylopectin + phosphate | - |
r | |
| starch + glucose 1-phosphate | soluble starch | Chlorella vulgaris | starch + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Chlorella vulgaris | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
ADPglucose | synthesis | Chlorella vulgaris | |
| 0.11 | - |
ADPglucose | phosphorolysis | Chlorella vulgaris | |
| 0.15 | - |
UDPglucose | synthesis | Chlorella vulgaris | |
| 0.51 | - |
UDPglucose | phosphorolysis | Chlorella vulgaris | |
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